Differentiation of neuroblastoma, glioma, and hybrid cells in culture as measured by the synthesis of specific protein species: Evidence for neuroblast-glioblast reciprocal genetic regulation

R. N. Rosenberg, C. K. Vance, M. Morrison, N. Prashad, J. Meyne, F. Baskin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Protein species from differentiating neuroblastoma, glioma, and hybrid neuroblastoma-glioma cell lines in cell culture were separated and identified initially in the first dimension by the use of isoelectric focusing gels and were further separated in the second dimension by SDS-acrylamide gels. There were two main classes of proteins identified: proteins which were dominantly expressed in neuroblastoma and also in hybrid cell cultures, and proteins which were expressed in glioma and also hybrid cells cultures. In general, proteins were identified which were significantly expressed in neuroblastoma cells and much reduced in glioma cultures, and also conversely so. The hybrid cell line expressed many of the neuroblastoma-type proteins and relatively fewer of the glioma type proteins. A specific protein species (z) was identified in hybrid cells and was not present in either parental neuroblastoma or glioma cultures. Protein z was expressed however by the co-culturing of neuroblastoma and glioma cells suggesting its induction is dependent on a soluble factor. Protein z in hybrid cells was demonstrated in both stained gels and by autoradiography. Chromosome analysis of hybrid cells confirmed the presence of both rat and mouse chromosomes. It is suggested that similar neuronal-glial interaction may be functional in the intact brain, and that similar reciprocal modulation between neurons and glia may be a central mechanism of differentiation in the nervous system.

Original languageEnglish (US)
Pages (from-to)1343-1355
Number of pages13
JournalJournal of Neurochemistry
Volume30
Issue number6
StatePublished - 1978

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Hybrid Cells
Neuroblastoma
Glioma
Cell Culture Techniques
Proteins
Cell culture
Gels
Chromosomes
Neuroglia
Cells
Cell Line
Acrylamide
Isoelectric Focusing
Neurology
Autoradiography
Nervous System
Neurons
Rats
Brain
Modulation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Differentiation of neuroblastoma, glioma, and hybrid cells in culture as measured by the synthesis of specific protein species : Evidence for neuroblast-glioblast reciprocal genetic regulation. / Rosenberg, R. N.; Vance, C. K.; Morrison, M.; Prashad, N.; Meyne, J.; Baskin, F.

In: Journal of Neurochemistry, Vol. 30, No. 6, 1978, p. 1343-1355.

Research output: Contribution to journalArticle

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