Differently anchored influenza hemagglutinin mutants display distinct interaction dynamics with mutual rafts

Dmitry E. Shvartsman, Mariana Kotler, Renee D. Tall, Michael G. Roth, Yoav I. Henis

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Lipid rafts play important roles in cellular functions through concentrating or sequestering membrane proteins. This requires proteins to differ in the stability of their interactions with lipid rafts. However, knowledge of the dynamics of membrane protein-raft interactions is lacking. We employed FRAP to measure in live cells the lateral diffusion of influenza hemagglutinin (HA) proteins that differ in raft association. This approach can detect weak interactions with rafts not detectable by biochemical methods. Wild-type (wt) HA and glycosylphosphatidylinositol (GPI)-anchored HA (BHA-PI) diffused slower than a nonraft HA mutant, but became equal to the latter after cholesterol depletion. When antigenically distinct BHA-PI and wt HA were coexpressed, aggregation of BHA-PI into immobile patches reduced wt HA diffusion rate, suggesting transient interactions with BHA-PI raft patches. Conversely, patching wt HA reduced the mobile fraction of BHA-PI, indicating stable interactions with wt HA patches. Thus, the anchoring mode determines protein-raft interaction dynamics. GPI-anchored and transmembrane proteins can share the same rafts, and different proteins can interact stably or transiently with the same raft domains.

Original languageEnglish (US)
Pages (from-to)879-888
Number of pages10
JournalJournal of Cell Biology
Volume163
Issue number4
DOIs
StatePublished - Nov 24 2003

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Hemagglutinins
Human Influenza
Butylated Hydroxyanisole
Glycosylphosphatidylinositols
Proteins
Membrane Proteins
Lipids
Cholesterol

Keywords

  • Fluorescence
  • Influenza hemagglutinin
  • Lateral diffusion
  • Photobleaching
  • Rafts

ASJC Scopus subject areas

  • Cell Biology

Cite this

Differently anchored influenza hemagglutinin mutants display distinct interaction dynamics with mutual rafts. / Shvartsman, Dmitry E.; Kotler, Mariana; Tall, Renee D.; Roth, Michael G.; Henis, Yoav I.

In: Journal of Cell Biology, Vol. 163, No. 4, 24.11.2003, p. 879-888.

Research output: Contribution to journalArticle

Shvartsman, Dmitry E. ; Kotler, Mariana ; Tall, Renee D. ; Roth, Michael G. ; Henis, Yoav I. / Differently anchored influenza hemagglutinin mutants display distinct interaction dynamics with mutual rafts. In: Journal of Cell Biology. 2003 ; Vol. 163, No. 4. pp. 879-888.
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