Diffuse vesicular distribution of Rab3D in the polarized neuroendocrine cell line AtT-20

Alberto M. Martelli, Renato Bareggi, Giovanna Baldimi, Philipp E. Scherer, Harvey F. Lodish, Giulia Baldini

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalFEBS Letters
Volume368
Issue number2
DOIs
StatePublished - Jul 17 1995

Keywords

  • AtT-20 endocrine cell line
  • Low molecular weight GTP-binding protein
  • Regulated secretion

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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