TY - JOUR
T1 - Diffuse vesicular distribution of Rab3D in the polarized neuroendocrine cell line AtT-20
AU - Martelli, Alberto M.
AU - Bareggi, Renato
AU - Baldimi, Giovanna
AU - Scherer, Philipp E.
AU - Lodish, Harvey F.
AU - Baldini, Giulia
N1 - Funding Information:
Acknowledgments: We thank R. Jahn for providing the anti-Rab3A antibody. This research was supported in part by Columbia University start-up funds. P.E.S. was supported by a fellowship from the Swiss National Science Foundation.
PY - 1995/7/17
Y1 - 1995/7/17
N2 - The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.
AB - The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.
KW - AtT-20 endocrine cell line
KW - Low molecular weight GTP-binding protein
KW - Regulated secretion
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U2 - 10.1016/0014-5793(95)00671-U
DO - 10.1016/0014-5793(95)00671-U
M3 - Article
C2 - 7628619
AN - SCOPUS:0029016988
SN - 0014-5793
VL - 368
SP - 271
EP - 275
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -