Digital image acquisition in in vivo confocal microscopy

Walter M Petroll; Harrison D Cavanagh; M. A. Lemp; P. M. Andrews

doi:10.1111/j.1365-2818.1992.tb04305.x

Digital image acquisition in in vivo confocal microscopy

Walter M Petroll, Harrison D Cavanagh, M. A. Lemp, P. M. Andrews

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

A flexible system for the real‐time acquisition of in vivo images has been developed. Images are generated using a tandem scanning confocal microscope interfaced to a low‐light‐level camera. The video signal from the camera is digitized and stored using a Gould image processing system with a real‐time digital disk (RTDD). The RTDD can store up to 3200 512 times 512 pixel images at video rates (30 images s⁻¹). Images can be input directly from the camera during the study, or off‐line from a Super VHS video recorder. Once a segment of experimental interest is digitized onto the RTDD, the user can interactively step through the images, average stable sequences, and identify candidates for further processing and analysis. Examples of how this system can be used to study the physiology of various organ systems in vivo are presented. 1992 Blackwell Science Ltd

Original language	English (US)
Pages (from-to)	61-69
Number of pages	9
Journal	Journal of Microscopy
Volume	165
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2818.1992.tb04305.x
State	Published - Jan 1992

Keywords

Confocal microscopy
image acquisition
in vivo
real‐time

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

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10.1111/j.1365-2818.1992.tb04305.x

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AU - Cavanagh, Harrison D

AU - Lemp, M. A.

AU - Andrews, P. M.

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KW - real‐time

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Digital image acquisition in in vivo confocal microscopy

Abstract

Keywords

ASJC Scopus subject areas

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