TY - JOUR
T1 - Digital plasmonic nanobubble detection for rapid and ultrasensitive virus diagnostics
AU - Liu, Yaning
AU - Ye, Haihang
AU - Huynh, Hoang Dinh
AU - Xie, Chen
AU - Kang, Peiyuan
AU - Kahn, Jeffrey S.
AU - Qin, Zhenpeng
N1 - Funding Information:
This research is partially supported by the National Institutes of Health (NIH) grants R21AI140462 and R01AI151374 and the CDMRP Peer Reviewed Medical Research Program Discovery Award PR192581 to Z.Q. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. We are grateful to the undergrad UT design team (Marisa Romero, Adrienne Phillips, Vinit Sheth, John Yoo, Michelle Chang, Austin Daneman) for helping with benchtop device assembly. We would like to thank Dr. Abdullah Bayram for useful discussions in further optimization of the optofluidic system to improve the sampling efficiency for the DIAMOND technique. We also thank Dr. Ruth Levitz and Xiaoqing Li for providing insightful suggestions for RSV quantification.
Funding Information:
This research is partially supported by the National Institutes of Health (NIH) grants R21AI140462 and R01AI151374 and the CDMRP Peer Reviewed Medical Research Program Discovery Award PR192581 to Z.Q. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. We are grateful to the undergrad UT design team (Marisa Romero, Adrienne Phillips, Vinit Sheth, John Yoo, Michelle Chang, Austin Daneman) for helping with benchtop device assembly. We would like to thank Dr. Abdullah Bayram for useful discussions in further optimization of the optofluidic system to improve the sampling efficiency for the DIAMOND technique. We also thank Dr. Ruth Levitz and Xiaoqing Li for providing insightful suggestions for RSV quantification.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Rapid and sensitive diagnostics of infectious diseases is an urgent and unmet need as evidenced by the COVID-19 pandemic. Here, we report a strategy, based on DIgitAl plasMONic nanobubble Detection (DIAMOND), to address this need. Plasmonic nanobubbles are transient vapor bubbles generated by laser heating of plasmonic nanoparticles (NPs) and allow single-NP detection. Using gold NPs as labels and an optofluidic setup, we demonstrate that DIAMOND achieves compartment-free digital counting and works on homogeneous immunoassays without separation and amplification steps. DIAMOND allows specific detection of respiratory syncytial virus spiked in nasal swab samples and achieves a detection limit of ~100 PFU/mL (equivalent to 1 RNA copy/µL), which is competitive with digital isothermal amplification for virus detection. Therefore, DIAMOND has the advantages including one-step and single-NP detection, direct sensing of intact viruses at room temperature, and no complex liquid handling, and is a platform technology for rapid and ultrasensitive diagnostics.
AB - Rapid and sensitive diagnostics of infectious diseases is an urgent and unmet need as evidenced by the COVID-19 pandemic. Here, we report a strategy, based on DIgitAl plasMONic nanobubble Detection (DIAMOND), to address this need. Plasmonic nanobubbles are transient vapor bubbles generated by laser heating of plasmonic nanoparticles (NPs) and allow single-NP detection. Using gold NPs as labels and an optofluidic setup, we demonstrate that DIAMOND achieves compartment-free digital counting and works on homogeneous immunoassays without separation and amplification steps. DIAMOND allows specific detection of respiratory syncytial virus spiked in nasal swab samples and achieves a detection limit of ~100 PFU/mL (equivalent to 1 RNA copy/µL), which is competitive with digital isothermal amplification for virus detection. Therefore, DIAMOND has the advantages including one-step and single-NP detection, direct sensing of intact viruses at room temperature, and no complex liquid handling, and is a platform technology for rapid and ultrasensitive diagnostics.
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U2 - 10.1038/s41467-022-29025-w
DO - 10.1038/s41467-022-29025-w
M3 - Article
C2 - 35354801
AN - SCOPUS:85127273693
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1687
ER -