Dimethylenastron suppresses human pancreatic cancer cell migration and invasion in vitro via allosteric inhibition of mitotic kinesin Eg5

Xiao Dong Sun, Xing Juan Shi, Xiao Ou Sun, You Guang Luo, Xiao Jing Wu, Chang Fu Yao, Hai Yang Yu, Deng Wen Li, Min Liu, Jun Zhou

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Aim: The mitotic kinesin Eg5 plays a critical role in bipolar spindle assembly, and its inhibitors have shown impressive anticancer activity in preclinical studies. This study was undertaken to investigate the effect of dimethylenastron, a specific inhibitor of Eg5, on the migration and invasion of pancreatic cancer cells. Methods: Human pancreatic cancer cell lines PANC1, EPP85, BxPC3, CFPAC1, and AsPAC1 were used. Eg5 expression was examined using immunofluorescence microscopy. Cell migration and invasion were analyzed with wound healing and transwell assays. Cell proliferation was examined using sulforhodamine B and MTT assays. The binding of dimethylenastron to Eg5 was analyzed with a molecular modeling study, and the ADP release rate was examined with the MANT-ADP reagent. Results: Eg5 expression was 9-16-fold up-regulated in the 5 pancreatic cancer cell lines. Treatment of PANC1 pancreatic cancer cells with dimethylenastron (3 and 10 μmol/L) for 24 h suppressed the migratory ability of the cancer cells in a concentration-dependent manner. The invasion ability of the cancer cells was also reduced by the treatment. However, treatment of PANC1 cells with dimethylenastron (3 and 10 μmol/L) for 24 h had no detectable effect on their proliferation, which was inhibited when the cancer cells were treated with the drug for 72 h. Molecular modeling study showed that dimethylenastron could allosterically inhibit the motor domain ATPase of Eg5 by decreasing the rate of ADP release. Conclusion: Dimethylenastron inhibits the migration and invasion of PANC1 pancreatic cancer cells, independent of suppressing the cell proliferation. The findings provide a novel insight into the mechanisms of targeting Eg5 for pancreatic cancer chemotherapy.

Original languageEnglish (US)
Pages (from-to)1543-1548
Number of pages6
JournalActa Pharmacologica Sinica
Volume32
Issue number12
DOIs
StatePublished - Dec 2011
Externally publishedYes

Keywords

  • Eg5
  • cell invasion
  • cell migration
  • cell proliferation
  • dimethylenastron
  • kinesin
  • molecular modeling
  • pancreatic cancer

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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