Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium: Identification of a new bisdiphospho-myo-inositol tetrakisphosphate

Tim Laussmann; André Hansen; Komandla Malla Reddy; K. Kishta Reddy; J R Falck; Günter Vogel

doi:10.1016/S0014-5793(98)00329-9

Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium: Identification of a new bisdiphospho-myo-inositol tetrakisphosphate

Tim Laussmann, André Hansen, Komandla Malla Reddy, K. Kishta Reddy, J R Falck, Günter Vogel

Biochemistry

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The two major diphospho inositol phosphates from the axenic strain Dictyostelium discoideum AX2 were previously investigated and identified as 6-PP-InsP₅ and 5,6-bis-PP-InsP₁. In order to examine whether these findings are representative of Dictyostelids in general, five non-axenic wild-type species of Dictyostelium and two of Polysphondylium were studied. It was found that all of the Dictyostelium species exhibit similar patterns of diphospho inositol phosphates. By contrast, both of the Polysphondylium species contain 5-PP-InsP₅ as the predominant isomer. Besides 5,6-bis-PP- InsP₄, a new bis-PP-InsP₄ was detected in Polysphondylium. This compound is either 1,5-bis-PP-InsP₄ or its corresponding enantiomer 3,5-bis-PP-InsP₅. The structures were elucidated by two-dimensional ¹H^-1H and ¹H-³¹P NMR analysis. Additionally, they were confirmed using a specific 6-PP-InsP₅-5- kinase from D. discoideum AX2 as an enantio-specific tool and enantiomerically pure reference standards.

Original language	English (US)
Pages (from-to)	145-150
Number of pages	6
Journal	FEBS Letters
Volume	426
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(98)00329-9
State	Published - Apr 10 1998

Keywords

Dictyostelium
Kinase
Myo-inositol
Polysphondylium

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00329-9

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title = "Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium: Identification of a new bisdiphospho-myo-inositol tetrakisphosphate",

abstract = "The two major diphospho inositol phosphates from the axenic strain Dictyostelium discoideum AX2 were previously investigated and identified as 6-PP-InsP5 and 5,6-bis-PP-InsP1. In order to examine whether these findings are representative of Dictyostelids in general, five non-axenic wild-type species of Dictyostelium and two of Polysphondylium were studied. It was found that all of the Dictyostelium species exhibit similar patterns of diphospho inositol phosphates. By contrast, both of the Polysphondylium species contain 5-PP-InsP5 as the predominant isomer. Besides 5,6-bis-PP- InsP4, a new bis-PP-InsP4 was detected in Polysphondylium. This compound is either 1,5-bis-PP-InsP4 or its corresponding enantiomer 3,5-bis-PP-InsP5. The structures were elucidated by two-dimensional 1H-1H and 1H-31P NMR analysis. Additionally, they were confirmed using a specific 6-PP-InsP5-5- kinase from D. discoideum AX2 as an enantio-specific tool and enantiomerically pure reference standards.",

keywords = "Dictyostelium, Kinase, Myo-inositol, Polysphondylium",

author = "Tim Laussmann and Andr{\'e} Hansen and Reddy, {Komandla Malla} and Reddy, {K. Kishta} and Falck, {J R} and G{\"u}nter Vogel",

note = "Funding Information: We thank Dr. Reint Eujen and Dr. C. Michael Weisshuhn for initially setting up the 2D 1 H- 31 P NMR method. We are grateful for skilled technical assistance of Mrs. Ilka Moeller. This work was supported by the Deutsche Forschungsgemeinschaft (grant VO 348/2-2), by the Fonds der Chemischen Industrie and grant NIH GM31278.",

year = "1998",

month = apr,

day = "10",

doi = "10.1016/S0014-5793(98)00329-9",

language = "English (US)",

volume = "426",

pages = "145--150",

journal = "FEBS Letters",

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T1 - Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium

T2 - Identification of a new bisdiphospho-myo-inositol tetrakisphosphate

AU - Laussmann, Tim

AU - Hansen, André

AU - Reddy, Komandla Malla

AU - Reddy, K. Kishta

AU - Falck, J R

AU - Vogel, Günter

N1 - Funding Information: We thank Dr. Reint Eujen and Dr. C. Michael Weisshuhn for initially setting up the 2D 1 H- 31 P NMR method. We are grateful for skilled technical assistance of Mrs. Ilka Moeller. This work was supported by the Deutsche Forschungsgemeinschaft (grant VO 348/2-2), by the Fonds der Chemischen Industrie and grant NIH GM31278.

PY - 1998/4/10

Y1 - 1998/4/10

N2 - The two major diphospho inositol phosphates from the axenic strain Dictyostelium discoideum AX2 were previously investigated and identified as 6-PP-InsP5 and 5,6-bis-PP-InsP1. In order to examine whether these findings are representative of Dictyostelids in general, five non-axenic wild-type species of Dictyostelium and two of Polysphondylium were studied. It was found that all of the Dictyostelium species exhibit similar patterns of diphospho inositol phosphates. By contrast, both of the Polysphondylium species contain 5-PP-InsP5 as the predominant isomer. Besides 5,6-bis-PP- InsP4, a new bis-PP-InsP4 was detected in Polysphondylium. This compound is either 1,5-bis-PP-InsP4 or its corresponding enantiomer 3,5-bis-PP-InsP5. The structures were elucidated by two-dimensional 1H-1H and 1H-31P NMR analysis. Additionally, they were confirmed using a specific 6-PP-InsP5-5- kinase from D. discoideum AX2 as an enantio-specific tool and enantiomerically pure reference standards.

AB - The two major diphospho inositol phosphates from the axenic strain Dictyostelium discoideum AX2 were previously investigated and identified as 6-PP-InsP5 and 5,6-bis-PP-InsP1. In order to examine whether these findings are representative of Dictyostelids in general, five non-axenic wild-type species of Dictyostelium and two of Polysphondylium were studied. It was found that all of the Dictyostelium species exhibit similar patterns of diphospho inositol phosphates. By contrast, both of the Polysphondylium species contain 5-PP-InsP5 as the predominant isomer. Besides 5,6-bis-PP- InsP4, a new bis-PP-InsP4 was detected in Polysphondylium. This compound is either 1,5-bis-PP-InsP4 or its corresponding enantiomer 3,5-bis-PP-InsP5. The structures were elucidated by two-dimensional 1H-1H and 1H-31P NMR analysis. Additionally, they were confirmed using a specific 6-PP-InsP5-5- kinase from D. discoideum AX2 as an enantio-specific tool and enantiomerically pure reference standards.

KW - Dictyostelium

KW - Kinase

KW - Myo-inositol

KW - Polysphondylium

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

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Diphospho-myo-inositol phosphates in Dictyostelium and Polysphondylium: Identification of a new bisdiphospho-myo-inositol tetrakisphosphate

Abstract

Keywords

ASJC Scopus subject areas

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