Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity

Jian Qiao; Karim Ghani; Manuel Caruso

doi:10.1016/j.toxicon.2007.09.010

Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity

Jian Qiao, Karim Ghani, Manuel Caruso

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Diphtheria toxin (DT) ADP-rybosylates elongation factor-2 on a modified histidine residue called diphthamide, leading to a block in protein synthesis. CRM197 is a nontoxic DT mutant commonly used as a carrier for conjugate vaccines, and may have a potential as an anti-tumor agent. We now report that CRM197 expression is indeed toxic to cells, and inhibits protein synthesis. These results should be considered for future vaccine studies, and to investigate the mechanism of CRM197-mediated anti-tumor effect.

Original language	English (US)
Pages (from-to)	473-477
Number of pages	5
Journal	Toxicon
Volume	51
Issue number	3
DOIs	https://doi.org/10.1016/j.toxicon.2007.09.010
State	Published - Mar 1 2008

Keywords

ADP-rybosylation
CRM197
Diphtheria toxin
Elongation factor-2

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxicon.2007.09.010

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title = "Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity",

abstract = "Diphtheria toxin (DT) ADP-rybosylates elongation factor-2 on a modified histidine residue called diphthamide, leading to a block in protein synthesis. CRM197 is a nontoxic DT mutant commonly used as a carrier for conjugate vaccines, and may have a potential as an anti-tumor agent. We now report that CRM197 expression is indeed toxic to cells, and inhibits protein synthesis. These results should be considered for future vaccine studies, and to investigate the mechanism of CRM197-mediated anti-tumor effect.",

keywords = "ADP-rybosylation, CRM197, Diphtheria toxin, Elongation factor-2",

author = "Jian Qiao and Karim Ghani and Manuel Caruso",

note = "Funding Information: We are grateful to Pedro Otavio de Campos-Lima for a critical reading of the manuscript and we thank Lloyd Mitchell for providing the CRM197 gene. This study was supported with a grant from the Canadian Institute of Health Research (CIHR) (IC074582). M.C. is a Senior 1 Research Scholar of the Fonds de la Recherche en Sant{\'e} du Qu{\'e}bec (FRSQ).",

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doi = "10.1016/j.toxicon.2007.09.010",

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T1 - Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity

AU - Qiao, Jian

AU - Ghani, Karim

AU - Caruso, Manuel

N1 - Funding Information: We are grateful to Pedro Otavio de Campos-Lima for a critical reading of the manuscript and we thank Lloyd Mitchell for providing the CRM197 gene. This study was supported with a grant from the Canadian Institute of Health Research (CIHR) (IC074582). M.C. is a Senior 1 Research Scholar of the Fonds de la Recherche en Santé du Québec (FRSQ).

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Diphtheria toxin (DT) ADP-rybosylates elongation factor-2 on a modified histidine residue called diphthamide, leading to a block in protein synthesis. CRM197 is a nontoxic DT mutant commonly used as a carrier for conjugate vaccines, and may have a potential as an anti-tumor agent. We now report that CRM197 expression is indeed toxic to cells, and inhibits protein synthesis. These results should be considered for future vaccine studies, and to investigate the mechanism of CRM197-mediated anti-tumor effect.

AB - Diphtheria toxin (DT) ADP-rybosylates elongation factor-2 on a modified histidine residue called diphthamide, leading to a block in protein synthesis. CRM197 is a nontoxic DT mutant commonly used as a carrier for conjugate vaccines, and may have a potential as an anti-tumor agent. We now report that CRM197 expression is indeed toxic to cells, and inhibits protein synthesis. These results should be considered for future vaccine studies, and to investigate the mechanism of CRM197-mediated anti-tumor effect.

KW - ADP-rybosylation

KW - CRM197

KW - Diphtheria toxin

KW - Elongation factor-2

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DO - 10.1016/j.toxicon.2007.09.010

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SN - 0041-0101

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JO - Toxicon

JF - Toxicon

IS - 3

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Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity

Abstract

Keywords

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