Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity

Jian Qiao, Karim Ghani, Manuel Caruso

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Diphtheria toxin (DT) ADP-rybosylates elongation factor-2 on a modified histidine residue called diphthamide, leading to a block in protein synthesis. CRM197 is a nontoxic DT mutant commonly used as a carrier for conjugate vaccines, and may have a potential as an anti-tumor agent. We now report that CRM197 expression is indeed toxic to cells, and inhibits protein synthesis. These results should be considered for future vaccine studies, and to investigate the mechanism of CRM197-mediated anti-tumor effect.

Original languageEnglish (US)
Pages (from-to)473-477
Number of pages5
JournalToxicon
Volume51
Issue number3
DOIs
StatePublished - Mar 1 2008

Fingerprint

Diphtheria Toxin
Protein Synthesis Inhibitors
Toxicity
Tumors
Peptide Elongation Factor 2
Conjugate Vaccines
Poisons
Histidine
Adenosine Diphosphate
Neoplasms
Proteins
Vaccines
CRM197 (non-toxic variant of diphtheria toxin)

Keywords

  • ADP-rybosylation
  • CRM197
  • Diphtheria toxin
  • Elongation factor-2

ASJC Scopus subject areas

  • Toxicology

Cite this

Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity. / Qiao, Jian; Ghani, Karim; Caruso, Manuel.

In: Toxicon, Vol. 51, No. 3, 01.03.2008, p. 473-477.

Research output: Contribution to journalArticle

Qiao, Jian ; Ghani, Karim ; Caruso, Manuel. / Diphtheria toxin mutant CRM197 is an inhibitor of protein synthesis that induces cellular toxicity. In: Toxicon. 2008 ; Vol. 51, No. 3. pp. 473-477.
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