Direct comparison of a genetically encoded sensor and small molecule indicator: Implications for quantification of cytosolic Zn2+

Yan Qin, Jose G. Miranda, Caitlin I. Stoddard, Kevin M. Dean, Domenico F. Galati, Amy E. Palmer

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Fluorescent sensors are powerful tools for visualizing and quantifying molecules and ions in living cells. A variety of small molecule and genetically encoded sensors have been developed for studying intracellular Zn2+ homeostasis and signaling, but no direct comparisons exist, making it challenging for researchers to identify the appropriate sensor for a given application. Here we directly compare the widely used small molecule probe FluoZin-3 and a genetically encoded sensor, ZapCY2. We demonstrate that, in contrast to FluoZin-3, ZapCY2 exhibits a well-defined cytosolic localization, provides estimates of Zn2+ concentration with little variability, does not perturb cytosolic Zn2+ levels, and exhibits rapid Zn 2+ response dynamics. ZapCY2 was used to measure Zn2+ concentrations in 5 different cell types, revealing higher cytosolic Zn 2+ levels in prostate cancer cells compared to normal prostate cells (although the total zinc is reduced in prostate cancer cells), suggesting distinct regulatory mechanisms.

Original languageEnglish (US)
Pages (from-to)2366-2371
Number of pages6
JournalACS chemical biology
Volume8
Issue number11
DOIs
StatePublished - Nov 15 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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