Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein

Michael K. Rosen, Toshio Yamazaki, Gerald D. Gish, Cyril M. Kay, Tony Pawson, Lewis E. Kay

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Abstract

Many signal transduction processes are mediated by the binding of Src-homology-2 (SH2) domains to phosphotyrosine (pTyr)-containing proteins 1. Although most SH2-pTyr interactions occur between two different types of molecules, some appear to involve only a single molecular type. It has been proposed that the enzymatic activity and substrate recognition of the Src-family kinases2-4, and the protein-binding and transforming activity of Crk-family adaptor proteins5, are regulated by intramolecular SH2-pTyr interactions. In addition, the DNA-binding activity of Stat transcription factors seems to be regulated by SH2-mediated homodimerization6. Here we examine the phosphorylated and non-phosphorylated forms of murine Crk II (p-mCrk and mCrk, respectively) 7-9 using a combination of physical techniques. The Crk protein contains a single SH2 domain and two SH3 domains in the order SH2-SH3-SH3. There is a tyrosine-phosphorylation site between the two SH3 domains at residue 221 which is phosphorylated in vivo by the Abl tyrosine kinase5. Using NMR spectroscopic analysis, we show here that the SH2 domain of purified p-mCrk is bound to pTyr, and by hydrodynamic measurements that the phosphorylated protein is monomeric. These results provide direct demonstration of an intramolecular SH2-pTyr inter-action in a signalling molecule.

Original languageEnglish (US)
Pages (from-to)477-479
Number of pages3
JournalNature
Volume374
Issue number6521
StatePublished - 1995

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Phosphotyrosine
src Homology Domains
Proteins
Tyrosine
Hydrodynamics
Protein Binding
Signal Transduction
Transcription Factors
Phosphorylation
DNA

ASJC Scopus subject areas

  • General

Cite this

Rosen, M. K., Yamazaki, T., Gish, G. D., Kay, C. M., Pawson, T., & Kay, L. E. (1995). Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein. Nature, 374(6521), 477-479.

Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein. / Rosen, Michael K.; Yamazaki, Toshio; Gish, Gerald D.; Kay, Cyril M.; Pawson, Tony; Kay, Lewis E.

In: Nature, Vol. 374, No. 6521, 1995, p. 477-479.

Research output: Contribution to journalArticle

Rosen, MK, Yamazaki, T, Gish, GD, Kay, CM, Pawson, T & Kay, LE 1995, 'Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein', Nature, vol. 374, no. 6521, pp. 477-479.
Rosen MK, Yamazaki T, Gish GD, Kay CM, Pawson T, Kay LE. Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein. Nature. 1995;374(6521):477-479.
Rosen, Michael K. ; Yamazaki, Toshio ; Gish, Gerald D. ; Kay, Cyril M. ; Pawson, Tony ; Kay, Lewis E. / Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein. In: Nature. 1995 ; Vol. 374, No. 6521. pp. 477-479.
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