Directing flux in glycan biosynthetic pathways with a small molecule switch

Jennifer J. Kohler, Jennifer L. Czlapinski, Scott T. Laughlin, Michael W. Schelle, Christopher L. De Graffenried, Carolyn R. Bertozzi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Directing sugar traffic. Golgi-resident glycosyltransferases act in concert to direct synthesis of a wide variety of cell-surface glycans. A re-engineered fucosyltransferase, whose activity is controlled with the small molecule rapamycin, can be used to divert flux away from production of one glycan (αGal) and toward synthesis of another (H antigen).

Original languageEnglish (US)
Pages (from-to)1455-1458
Number of pages4
JournalChemBioChem
Volume5
Issue number10
DOIs
StatePublished - Oct 4 2004

Fingerprint

Biosynthetic Pathways
Polysaccharides
Switches
Fucosyltransferases
Fluxes
Glycosyltransferases
Molecules
Sirolimus
Sugars
H antigen

Keywords

  • Carbohydrates
  • Cell surface
  • Chemical genetics
  • Glycosylation
  • Protein engineering

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kohler, J. J., Czlapinski, J. L., Laughlin, S. T., Schelle, M. W., De Graffenried, C. L., & Bertozzi, C. R. (2004). Directing flux in glycan biosynthetic pathways with a small molecule switch. ChemBioChem, 5(10), 1455-1458. https://doi.org/10.1002/cbic.200400156

Directing flux in glycan biosynthetic pathways with a small molecule switch. / Kohler, Jennifer J.; Czlapinski, Jennifer L.; Laughlin, Scott T.; Schelle, Michael W.; De Graffenried, Christopher L.; Bertozzi, Carolyn R.

In: ChemBioChem, Vol. 5, No. 10, 04.10.2004, p. 1455-1458.

Research output: Contribution to journalArticle

Kohler, JJ, Czlapinski, JL, Laughlin, ST, Schelle, MW, De Graffenried, CL & Bertozzi, CR 2004, 'Directing flux in glycan biosynthetic pathways with a small molecule switch', ChemBioChem, vol. 5, no. 10, pp. 1455-1458. https://doi.org/10.1002/cbic.200400156
Kohler JJ, Czlapinski JL, Laughlin ST, Schelle MW, De Graffenried CL, Bertozzi CR. Directing flux in glycan biosynthetic pathways with a small molecule switch. ChemBioChem. 2004 Oct 4;5(10):1455-1458. https://doi.org/10.1002/cbic.200400156
Kohler, Jennifer J. ; Czlapinski, Jennifer L. ; Laughlin, Scott T. ; Schelle, Michael W. ; De Graffenried, Christopher L. ; Bertozzi, Carolyn R. / Directing flux in glycan biosynthetic pathways with a small molecule switch. In: ChemBioChem. 2004 ; Vol. 5, No. 10. pp. 1455-1458.
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