Disabled-2 is a novel αIIb-integrin-binding protein that negatively regulates platelet-fibrinogen interactions and platelet aggregation

Chien Ling Huang, Ju Chien Cheng, Arnold Stern, Jer Tsong Hsieh, Chang Hui Liao, Ching Ping Tseng

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Platelet aggregation plays a pivotal role in the haemostatic process and is involved in the pathological counterpart of arterial thrombosis. We have shown that the adapter protein disabled-2 (DAB2) is expressed abundantly in platelets. In this study, DAB2 was found to distribute in the platelet α-granules and was released from the granular compartment upon platelet activation. The secreted DAB2 binds to the extracellular region of αIIbβ3 integrin on the platelet surface through the phosphotyrosine-binding domain. The DAB2-platelet interactions result in the inhibition of agonist-induced platelet aggregation with the exception of thrombin, a DAB2 protease that renders DAB2 inactive. Biochemical and mutational analysis revealed that the DAB2 cell-adhesion Arg-Gly-Asp (RGD) motif (amino acid residues 64-66) and the αIIb-integrin-fibrinogen-binding region (amino acid residues 171-464) are important for the binding of αIIb integrin with fibrinogen and provide a mechanism for DAB2 to inhibit platelet aggregation. Accordingly, the synthetic RGD-motif-containing DAB2 peptide PDARGDKM also elicited anti-platelet aggregation activity. These findings demonstrate for the first time that DAB2 is an αIIb-integrin-binding protein that plays a novel role in the control of platelet-fibrinogen interactions and platelet aggregation.

Original languageEnglish (US)
Pages (from-to)4420-4430
Number of pages11
JournalJournal of cell science
Volume119
Issue number21
DOIs
StatePublished - Nov 1 2006

Keywords

  • Disabled-2
  • Fibrinogen
  • Platelet aggregation
  • RGD motif
  • αIIbβ3 Integrin

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Disabled-2 is a novel αIIb-integrin-binding protein that negatively regulates platelet-fibrinogen interactions and platelet aggregation'. Together they form a unique fingerprint.

Cite this