Abstract
Background: The present studies determined the role of LIGHT on organ-specific cytokine and effector molecules in acute graft-versus-host disease. Methods: Cytokine and effector molecules were assessed by flow cytometry and quantitative PCR. Results: More CD4+ spleen cells (SpC) expressing interferon-γ (IFNγ) and interleukin-2 were noted in SpC isolated from lethally irradiated bm12 X B6 F1 recipients of B6 donor SpC and T cell-depleted bone marrow cells and control Adv-βgal than in transplant (bone marrow transplantation (BMT)) recipients who had received Adv-LTβR-Ig or Adv-herpes simplex virus entry mediator (HVEM)-Ig. IFNγ RNA levels from SpC, small intestines, and large intestines of control BMT recipients were significantly higher than those who had received Adv-HVEM-Ig. Granzyme B levels from SpC and small intestines of control BMT recipients were significantly higher than those that had received the Adv-LTβR-Ig. In contrast, BMT recipients of Adv-HVEM-Ig had lower granzyme A levels than controls in their large intestines. Discussion: LIGHT inhibition differentially affects cytokines and effector molecules in SpC, small intestines, and large intestines, implicating different organ-specific pathways.
Original language | English (US) |
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Pages (from-to) | 178-184 |
Number of pages | 7 |
Journal | Journal of Clinical Immunology |
Volume | 30 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2010 |
Keywords
- Cytokines
- Cytotoxic
- Graft-versus-host disease
- Rodent
- T cells
- Transplantation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology