Disruption of the actin cytoskeleton in yeast capping protein mutants

James F. Amatruda, John F. Cannon, Kelly Tatchell, Christopher Hug, John A. Cooper

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

CAPPING protein controls the addition of actin subunits to the barbed end of actin filaments and nucleates actin polymerization in vitro. Capping protein has been identified in all eukaryotic cells examined so far; it is a heterodimer with subunits of relative molecular masses 32,000-36,000 (α-subunit) and 28,000-32,000 (β-subunit)1,2. In skeletal muscle, capping protein (CapZ) probably binds the barbed ends of actin filaments at the Z line3. The in vivo role of this protein in non-muscle cells is not known. We report here the characterization of CAP2, the single gene encoding the β-subunit of capping protein in Saccharomyces cerevisiae. Yeast cells in which the CAP2 gene was disrupted by an insertion or a deletion had an abnormal actin distribution, including the loss of actin cables. The mutant cells were round and large, with a heterogeneous size distribution, and, although viable, grew more slowly than congenic wild-type cells. Chitin, a cell wall component restricted to the mother-bud junction in wild-type budding yeast, was found on the entire mother cell surface in the mutants. The phenotype of CAP2 disruption resembled that of temperature-sensitive mutations in the yeast actin gene ACT1 (ref. 4), indicating that capping protein regulates actin-filament distribution in vivo.

Original languageEnglish (US)
Pages (from-to)352-354
Number of pages3
JournalNature
Volume344
Issue number6264
StatePublished - Mar 22 1990

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Fungal Proteins
Actin Cytoskeleton
Actins
CapZ Actin Capping Protein
Actin Capping Proteins
Yeasts
Genes
Saccharomyces cerevisiae Proteins
Proteins
Saccharomycetales
Chitin
Eukaryotic Cells
Cellular Structures
Polymerization
Cell Wall
Skeletal Muscle
Stem Cells
Phenotype
Mutation
Temperature

ASJC Scopus subject areas

  • General

Cite this

Amatruda, J. F., Cannon, J. F., Tatchell, K., Hug, C., & Cooper, J. A. (1990). Disruption of the actin cytoskeleton in yeast capping protein mutants. Nature, 344(6264), 352-354.

Disruption of the actin cytoskeleton in yeast capping protein mutants. / Amatruda, James F.; Cannon, John F.; Tatchell, Kelly; Hug, Christopher; Cooper, John A.

In: Nature, Vol. 344, No. 6264, 22.03.1990, p. 352-354.

Research output: Contribution to journalArticle

Amatruda, JF, Cannon, JF, Tatchell, K, Hug, C & Cooper, JA 1990, 'Disruption of the actin cytoskeleton in yeast capping protein mutants', Nature, vol. 344, no. 6264, pp. 352-354.
Amatruda JF, Cannon JF, Tatchell K, Hug C, Cooper JA. Disruption of the actin cytoskeleton in yeast capping protein mutants. Nature. 1990 Mar 22;344(6264):352-354.
Amatruda, James F. ; Cannon, John F. ; Tatchell, Kelly ; Hug, Christopher ; Cooper, John A. / Disruption of the actin cytoskeleton in yeast capping protein mutants. In: Nature. 1990 ; Vol. 344, No. 6264. pp. 352-354.
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