Disruption of type III secretion in Salmonella enterica serovar typhimurium by external guide sequences

Jeffrey S. McKinney; Haifeng Zhang; Tomoko Kubori; Jorge E. Galán; Sidney Altman

doi:10.1093/nar/gkh219

Disruption of type III secretion in Salmonella enterica serovar typhimurium by external guide sequences

Jeffrey S. McKinney, Haifeng Zhang, Tomoko Kubori, Jorge E. Galán, Sidney Altman

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.

Original language	English (US)
Pages (from-to)	848-854
Number of pages	7
Journal	Nucleic acids research
Volume	32
Issue number	2
DOIs	https://doi.org/10.1093/nar/gkh219
State	Published - 2004

ASJC Scopus subject areas

Genetics

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title = "Disruption of type III secretion in Salmonella enterica serovar typhimurium by external guide sequences",

abstract = "The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.",

author = "McKinney, {Jeffrey S.} and Haifeng Zhang and Tomoko Kubori and Gal{\'a}n, {Jorge E.} and Sidney Altman",

note = "Funding Information: We thank Yukihiro Akeda for antibody against InvC and for the invC Salmonella mutant. This research was conducted while J.M. was a Pfizer postdoctoral fellow. The work was supported by an NIH research training program (NIH T32 AI07210-17), a Yale Children's Health Center Scholar Award and a Washington University Digestive Diseases Research Core Center Grant (DDRCC P30 DK52574) to J.M., and NIH grants AI030492 to J.E.G. and GM19422 to S.A.",

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doi = "10.1093/nar/gkh219",

language = "English (US)",

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pages = "848--854",

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AU - McKinney, Jeffrey S.

AU - Zhang, Haifeng

AU - Kubori, Tomoko

AU - Galán, Jorge E.

AU - Altman, Sidney

N1 - Funding Information: We thank Yukihiro Akeda for antibody against InvC and for the invC Salmonella mutant. This research was conducted while J.M. was a Pfizer postdoctoral fellow. The work was supported by an NIH research training program (NIH T32 AI07210-17), a Yale Children's Health Center Scholar Award and a Washington University Digestive Diseases Research Core Center Grant (DDRCC P30 DK52574) to J.M., and NIH grants AI030492 to J.E.G. and GM19422 to S.A.

PY - 2004

Y1 - 2004

N2 - The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.

AB - The type III secretion system involved in Salmonella enterica serovar Typhimurium invasion of host cells has been disrupted using inducibly expressed oligonucleotide external guide sequences (EGSs) complementary to invB or invC mRNA. These EGSs direct single site cleavage in these mRNAs by endogenous RNase P, and their expression in Salmonella results in invC mRNA and InvC protein depletion, decreased type III secretion and interference with host cell invasion. Comparison of these effects with those from studies of Salmonella invB and invC mutants suggests that invB EGSs have polar effects on invC mRNA.

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JO - Nucleic acids research

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Disruption of type III secretion in Salmonella enterica serovar typhimurium by external guide sequences

Abstract

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