Dissociation of clathrin coats coupled to the hydrolysis of ATP: Role of an uncoating ATPase

W. A. Braell, D. M. Schlossman, S. L. Schmid, J. E. Rothman

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

ATP hydrolysis was used to power the enzymatic release of clathrin from coated vesicles. The 70,000-mol-wt protein, purified on the basis of its ATP-dependent ability to disassemble clathrin cages, was found to possess a clathrin-dependent ATPase activity. Hydrolysis was specific for ATP; neither dATP nor other ribonucleotide triphosphates would either substitute for ATP or inhibit the hydrolysis of ATP in the presence of clathrin cages. The ATPase activity is elicited by clathrin in the form of assembled cages, but not by clathrin trimers, the product of cage disassembly. The 70,000-mol-wt polypeptide, but not clathrin, was labeled by ATP in photochemical cross-linking, indicating that the hydrolytic site for ATP resides on the uncoating protein. Conditions of low pH or high magnesium concentration uncouple ATP hydrolysis from clathrin release, as ATP is hydrolyzed although essentially no clathrin is released. This suggests that the recognition event triggering clathrin-dependent ATP hydrolysis occurs in the absence of clathrin release, and presumably precedes such release.

Original languageEnglish (US)
Pages (from-to)735-741
Number of pages7
JournalJournal of Cell Biology
Volume99
Issue number2
StatePublished - 1984

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Clathrin
Adenosine Triphosphatases
Hydrolysis
Adenosine Triphosphate
Clathrin-Coated Vesicles
Ribonucleotides
Magnesium
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dissociation of clathrin coats coupled to the hydrolysis of ATP : Role of an uncoating ATPase. / Braell, W. A.; Schlossman, D. M.; Schmid, S. L.; Rothman, J. E.

In: Journal of Cell Biology, Vol. 99, No. 2, 1984, p. 735-741.

Research output: Contribution to journalArticle

Braell, W. A. ; Schlossman, D. M. ; Schmid, S. L. ; Rothman, J. E. / Dissociation of clathrin coats coupled to the hydrolysis of ATP : Role of an uncoating ATPase. In: Journal of Cell Biology. 1984 ; Vol. 99, No. 2. pp. 735-741.
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