Distinct domains of complexin I differentially regulate neurotransmitter release

Mingshan Xue, Kerstin Reim, Xiaocheng Chen, Hsiao Tuan Chao, Hui Deng, Jose Rizo-Rey, Nils Brose, Christian Rosenmund

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Complexins constitute a family of four synaptic high-affinity SNARE complex-binding proteins. They positively regulate a late, post-priming step in Ca2+-triggered synchronous neurotransmitter release, but the underlying molecular mechanisms are unclear. We show here that SNARE complex binding of complexin I (CplxI) via its central α-helix is necessary but, unexpectedly, not sufficient for its key function in promoting neurotransmitter release. An accessory α-helix on the N-terminal side of the SNARE complex-binding region has an inhibitory effect on fast synaptic exocytosis, whereas sequences N-terminally adjacent to this helix facilitate Ca 2+-triggered release even in the absence of the Ca2+ sensor synaptotagmin-1. Our results indicate that distinct functional domains of CplxI differentially regulate synaptic exocytosis and that, through the interplay between these domains, CplxI carries out a crucial role in fine-tuning Ca2+-triggered fast neurotransmitter release.

Original languageEnglish (US)
Pages (from-to)949-958
Number of pages10
JournalNature Structural and Molecular Biology
Volume14
Issue number10
DOIs
StatePublished - Oct 2007

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SNARE Proteins
Neurotransmitter Agents
Exocytosis
Synaptotagmin I
Carrier Proteins
complexin I

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Distinct domains of complexin I differentially regulate neurotransmitter release. / Xue, Mingshan; Reim, Kerstin; Chen, Xiaocheng; Chao, Hsiao Tuan; Deng, Hui; Rizo-Rey, Jose; Brose, Nils; Rosenmund, Christian.

In: Nature Structural and Molecular Biology, Vol. 14, No. 10, 10.2007, p. 949-958.

Research output: Contribution to journalArticle

Xue, M, Reim, K, Chen, X, Chao, HT, Deng, H, Rizo-Rey, J, Brose, N & Rosenmund, C 2007, 'Distinct domains of complexin I differentially regulate neurotransmitter release', Nature Structural and Molecular Biology, vol. 14, no. 10, pp. 949-958. https://doi.org/10.1038/nsmb1292
Xue, Mingshan ; Reim, Kerstin ; Chen, Xiaocheng ; Chao, Hsiao Tuan ; Deng, Hui ; Rizo-Rey, Jose ; Brose, Nils ; Rosenmund, Christian. / Distinct domains of complexin I differentially regulate neurotransmitter release. In: Nature Structural and Molecular Biology. 2007 ; Vol. 14, No. 10. pp. 949-958.
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