@article{3fdde8f97d9c4fe58d9d2df79b7b913a,
title = "Distinct expression patterns of SULT2B1b in human prostate epithelium",
abstract = "Background: SULT2B1b (sulfotransferase family cytosolic 2B member 1b) catalyzes the sulfate conjugation of substrates such as cholesterol and oxysterols. Our laboratory has previously shown that SULT2B1b inhibition modulates androgen receptor signaling and induces apoptosis in prostate cancer cells. However, the functions of SULT2B1b in the prostate remain poorly understood. Methods: We characterized the expression pattern of SULT2B1b in human benign prostate hyperplasia (BPH) as well as prostate cancer to determine the relationship between SULT2B1b and prostate diseases, using immunohistochemistry, immunofluorescence staining, immunoblot, and real-time polymerase chain reaction. Results: SULT2B1b was strongly detected in the prostate epithelium but was absent in the stroma. Significantly lower SULT2B1b was found in primary cancer cells compared with adjacent normal epithelial cells. SULT2B1b further decreased in metastatic cancer cells. Most interestingly, we found, for the first time, that SULT2B1b was much more concentrated in the luminal layer than in the basal layer in both normal prostate and BPH samples. The stronger presence of SULT2B1b in luminal epithelial cells was confirmed by costaining with luminal and basal markers and in sorted paired luminal and basal cells. SULT2B1b expression was induced with prostate organoid differentiation. Conclusions: SULT2B1b inversely correlates with prostate cancer status, with the highest level in the normal epithelium and lowest in the advanced metastatic prostate cancer. Furthermore, SULT2B1b is mostly located within the luminal layer of the prostate epithelium, suggesting that it may be implicated in luminal differentiation.",
keywords = "basal, benign prostate hyperplasia, luminal, prostate cancer",
author = "Jiang Yang and Broman, {Meaghan M.} and Cooper, {Paula O.} and Lanman, {Nadia A.} and Strand, {Douglas W.} and Colm Morrissey and Ratliff, {Timothy L.}",
note = "Funding Information: These studies were supported by the Department of Defense Prostate Cancer Program (W81XWH-14-1-058830), the Purdue University Center for Cancer Research (NIH grant P30 CA023168), and the Walther Cancer Foundation. The authors acknowledge the assistance of Flow Cytometry and Cell Separation Facility of the Bindley Bioscience Center and Purdue University Histology Research Laboratory, both of which are core facilities of the NIH-funded Indiana Clinical and Translational Science Institute. The authors also thank the patients and their families, Drs. Celestia Higano, Evan Yu, Pete Nelson, Bruce Montgomery, Elahe Mostaghel, Paul Lange, Bill Ellis, Dan Lin, Xiaotun Zhang, Martine Roudier, Lawrence True, Robert Vessella, and the rapid autopsy teams for their contributions to the University of Washington Medical Center Prostate Cancer Donor Rapid Autopsy Program. The material from the University of Washington was the result of work supported by resources by the Pacific Northwest Prostate Cancer SPORE (P50CA97186), the PO1 NIH grant (PO1CA163227), and the Institute for Prostate Cancer Research. Funding Information: These studies were supported by the Department of Defense Prostate Cancer Program (W81XWH‐14‐1‐058830), the Purdue University Center for Cancer Research (NIH grant P30 CA023168), and the Walther Cancer Foundation. The authors acknowledge the assistance of Flow Cytometry and Cell Separation Facility of the Bindley Bioscience Center and Purdue University Histology Research Laboratory, both of which are core facilities of the NIH‐funded Indiana Clinical and Translational Science Institute. The authors also thank the patients and their families, Drs. Celestia Higano, Evan Yu, Pete Nelson, Bruce Montgomery, Elahe Mostaghel, Paul Lange, Bill Ellis, Dan Lin, Xiaotun Zhang, Martine Roudier, Lawrence True, Robert Vessella, and the rapid autopsy teams for their contributions to the University of Washington Medical Center Prostate Cancer Donor Rapid Autopsy Program. The material from the University of Washington was the result of work supported by resources by the Pacific Northwest Prostate Cancer SPORE (P50CA97186), the PO1 NIH grant (PO1CA163227), and the Institute for Prostate Cancer Research. Publisher Copyright: {\textcopyright} 2019 Wiley Periodicals, Inc.",
year = "2019",
month = aug,
day = "1",
doi = "10.1002/pros.23829",
language = "English (US)",
volume = "79",
pages = "1256--1266",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "11",
}