Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel

Chunguang Kong, Weizhong Zeng, Sheng Ye, Liping Chen, David Bryant Sauer, Yeeling Lam, Mehabaw Getahun Derebe, Youxing Jiang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD+ activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.

Original languageEnglish (US)
Article numbere00184
JournaleLife
Volume2012
Issue number1
DOIs
StatePublished - Dec 13 2012

Fingerprint

Ligand-Gated Ion Channels
Geobacter
Ligands
Mechanics
NAD
Adenosine Diphosphate
Ions
Crystal structure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel. / Kong, Chunguang; Zeng, Weizhong; Ye, Sheng; Chen, Liping; Sauer, David Bryant; Lam, Yeeling; Derebe, Mehabaw Getahun; Jiang, Youxing.

In: eLife, Vol. 2012, No. 1, e00184, 13.12.2012.

Research output: Contribution to journalArticle

Kong, C, Zeng, W, Ye, S, Chen, L, Sauer, DB, Lam, Y, Derebe, MG & Jiang, Y 2012, 'Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel', eLife, vol. 2012, no. 1, e00184. https://doi.org/10.7554/eLife.00184
Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y et al. Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel. eLife. 2012 Dec 13;2012(1). e00184. https://doi.org/10.7554/eLife.00184
Kong, Chunguang ; Zeng, Weizhong ; Ye, Sheng ; Chen, Liping ; Sauer, David Bryant ; Lam, Yeeling ; Derebe, Mehabaw Getahun ; Jiang, Youxing. / Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel. In: eLife. 2012 ; Vol. 2012, No. 1.
@article{1ba43a05d1764fe69c91d13d26b8337f,
title = "Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel",
abstract = "The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD+ activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.",
author = "Chunguang Kong and Weizhong Zeng and Sheng Ye and Liping Chen and Sauer, {David Bryant} and Yeeling Lam and Derebe, {Mehabaw Getahun} and Youxing Jiang",
year = "2012",
month = "12",
day = "13",
doi = "10.7554/eLife.00184",
language = "English (US)",
volume = "2012",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "1",

}

TY - JOUR

T1 - Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel

AU - Kong, Chunguang

AU - Zeng, Weizhong

AU - Ye, Sheng

AU - Chen, Liping

AU - Sauer, David Bryant

AU - Lam, Yeeling

AU - Derebe, Mehabaw Getahun

AU - Jiang, Youxing

PY - 2012/12/13

Y1 - 2012/12/13

N2 - The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD+ activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.

AB - The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD+ activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.

UR - http://www.scopus.com/inward/record.url?scp=84881538221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881538221&partnerID=8YFLogxK

U2 - 10.7554/eLife.00184

DO - 10.7554/eLife.00184

M3 - Article

C2 - 23240087

AN - SCOPUS:84881538221

VL - 2012

JO - eLife

JF - eLife

SN - 2050-084X

IS - 1

M1 - e00184

ER -