Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel

Chunguang Kong, Weizhong Zeng, Sheng Ye, Liping Chen, David Bryant Sauer, Yeeling Lam, Mehabaw Getahun Derebe, Youxing Jiang

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17 Scopus citations

Abstract

The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K+ channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K+ channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD+ activate the GsuK channel, whereas Ca2+ serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K+ channels.

Original languageEnglish (US)
Article numbere00184
JournaleLife
Volume2012
Issue number1
DOIs
StatePublished - Dec 13 2012

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kong, C., Zeng, W., Ye, S., Chen, L., Sauer, D. B., Lam, Y., Derebe, M. G., & Jiang, Y. (2012). Distinct gating mechanisms revealed by the structures of a multi-ligand gated K+ channel. eLife, 2012(1), [e00184]. https://doi.org/10.7554/eLife.00184