TY - JOUR
T1 - Distinct immunophenotype of early T-cell progenitors in T lymphoblastic leukemia/lymphoma may predict FMS-like tyrosine kinase 3 mutations
AU - Zaremba, Charles M.
AU - Oliver, Dwight
AU - Cavalier, Maryellen
AU - Fuda, Franklin
AU - Karandikar, Nitin J.
AU - Chen, Weina
PY - 2012/1
Y1 - 2012/1
N2 - FMS-like tyrosine kinase 3 (FLT3) mutation in T lymphoblastic leukemia/lymphoma (T-LL) is rare (∼4%) and reported only in cases with CD117 expression. This study aimed to identify the immunophenotypic features that may predict FLT3 mutations. We report 3 (43%) of 7 CD117 + T-LL cases harboring FLT3-internal tandem duplication mutation. Compared with 4 FLT3-unmutated cases, all 3 FLT3-mutated cases had a distinct immunophenotype (CD1a -/CD2 +/CD7 +/CD34 +/CD117 uniform+/Tdt +) corresponding to the stage of earliest thymic T-cell progenitors possessing myeloid lineage potential. Indeed, all FLT3-mutated T-LL cases expressed myeloperoxidase on a very small subset of blasts and, thus, may be further considered a mixed phenotype acute leukemia, T/myeloid, by the 2008 World Health Organization classification scheme. We conclude that this unique immunophenotype (CD1a -/CD2 +/CD7 +/CD34 +/CD117 +/Tdt +) is a better predictor of FLT3 mutation than sole CD117 expression.
AB - FMS-like tyrosine kinase 3 (FLT3) mutation in T lymphoblastic leukemia/lymphoma (T-LL) is rare (∼4%) and reported only in cases with CD117 expression. This study aimed to identify the immunophenotypic features that may predict FLT3 mutations. We report 3 (43%) of 7 CD117 + T-LL cases harboring FLT3-internal tandem duplication mutation. Compared with 4 FLT3-unmutated cases, all 3 FLT3-mutated cases had a distinct immunophenotype (CD1a -/CD2 +/CD7 +/CD34 +/CD117 uniform+/Tdt +) corresponding to the stage of earliest thymic T-cell progenitors possessing myeloid lineage potential. Indeed, all FLT3-mutated T-LL cases expressed myeloperoxidase on a very small subset of blasts and, thus, may be further considered a mixed phenotype acute leukemia, T/myeloid, by the 2008 World Health Organization classification scheme. We conclude that this unique immunophenotype (CD1a -/CD2 +/CD7 +/CD34 +/CD117 +/Tdt +) is a better predictor of FLT3 mutation than sole CD117 expression.
KW - Biphenotypic leukemia
KW - CD117
KW - FLT3 mutation
KW - Immunophenotype
KW - T lymphoblastic leukemia
UR - http://www.scopus.com/inward/record.url?scp=84856023442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856023442&partnerID=8YFLogxK
U2 - 10.1016/j.anndiagpath.2011.07.005
DO - 10.1016/j.anndiagpath.2011.07.005
M3 - Article
C2 - 22050963
AN - SCOPUS:84856023442
SN - 1092-9134
VL - 16
SP - 16
EP - 20
JO - Annals of Diagnostic Pathology
JF - Annals of Diagnostic Pathology
IS - 1
ER -