Distinct Interactions Select and Maintain a Specific Cell Fate

Andreas Doncic, Melody Falleur-Fettig, Jan M. Skotheim

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The ability to specify and maintain discrete cell fates is essential for development. However, the dynamics underlying selection and stability of distinct cell types remain poorly understood. Here, we provide a quantitative single-cell analysis of commitment dynamics during the mating-mitosis switch in budding yeast. Commitment to division corresponds precisely to activating the G1 cyclin positive feedback loop in competition with the cyclin inhibitor Far1. Cyclin-dependent phosphorylation and inhibition of the mating pathway scaffold Ste5 are required to ensure exclusive expression of the mitotic transcriptional program after cell cycle commitment. Failure to commit exclusively results in coexpression of both cell cycle and pheromone-induced genes, and a morphologically mixed inviable cell fate. Thus, specification and maintenance of a cellular state are performed by distinct interactions, which are likely a consequence of disparate reaction rates and may be a general feature of the interlinked regulatory networks responsible for selecting cell fates.

Original languageEnglish (US)
Pages (from-to)528-539
Number of pages12
JournalMolecular Cell
Volume43
Issue number4
DOIs
StatePublished - Aug 19 2011

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Cyclins
Cell Cycle
Cyclin G1
Single-Cell Analysis
Saccharomycetales
Pheromones
Mitosis
Maintenance
Phosphorylation
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Distinct Interactions Select and Maintain a Specific Cell Fate. / Doncic, Andreas; Falleur-Fettig, Melody; Skotheim, Jan M.

In: Molecular Cell, Vol. 43, No. 4, 19.08.2011, p. 528-539.

Research output: Contribution to journalArticle

Doncic, Andreas ; Falleur-Fettig, Melody ; Skotheim, Jan M. / Distinct Interactions Select and Maintain a Specific Cell Fate. In: Molecular Cell. 2011 ; Vol. 43, No. 4. pp. 528-539.
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