Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia

Ryan K. Butler, Sarah Ehling, Megan Barbar, Jess Thomas, Mary A. Hughes, Charles E. Smith, Vladimir M. Pogorelov, Dipendra K. Aryal, William C. Wetsel, B. Duncan X. Lascelles

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fear-conditioned analgesia (FCA) is modulated by brain areas involved in the descending inhibitory pain pathway such as the basolateral (BLA) and central amygdala (CEA). The BLA contains Ca2+/calmodulin-dependent protein kinase II (CaMKII) and parvalbumin (PV) neurons. CEA neurons are primarily inhibitory (GABAergic) that comprise enkephalin (ENK) interneurons and corticotropin-releasing factor (CRF) – neurons that project to the periaqueductal grey. The purpose of our experiment was to determine the pattern of activation of CaMKII/PV and ENK/CRF neurons following the expression of acute pain, conditioned fear, and FCA. A significant reduction was observed in nociceptive behaviors in mice re-exposed to a contextually-aversive environment. Using NeuN and cFos as markers for activated neurons, CaMKII, PV, ENK, or CRF were used to identify neuronal subtypes. We find that mice expressing conditioned fear displayed an increase in c-Fos/CaMKII co-localization in the lateral amygdala and BLA compared to controls. Additionally a significant increase in cFos/CRF co-localization was observed in mice expressing FCA. These results show that amygdala processing of conditioned contextual aversive, nociceptive, and FCA behaviors involve different neuronal phenotypes and neural circuits between, within, and from various amygdala nuclei. This information will be important in developing novel therapies for treating pain and emotive disorders in humans.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalNeuroscience Letters
Volume661
DOIs
StatePublished - Nov 20 2017
Externally publishedYes

Fingerprint

Acute Pain
Analgesia
Fear
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Corticotropin-Releasing Hormone
Parvalbumins
Enkephalins
Neurons
Population
Amygdala
Periaqueductal Gray
Somatoform Disorders
Interneurons
Central Amygdaloid Nucleus
Basolateral Nuclear Complex
Phenotype
Pain
Brain

Keywords

  • Amygdala
  • cFos
  • Contextual fear
  • Fear-conditioned analgesia
  • Neural circuits
  • Nociception

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia. / Butler, Ryan K.; Ehling, Sarah; Barbar, Megan; Thomas, Jess; Hughes, Mary A.; Smith, Charles E.; Pogorelov, Vladimir M.; Aryal, Dipendra K.; Wetsel, William C.; Lascelles, B. Duncan X.

In: Neuroscience Letters, Vol. 661, 20.11.2017, p. 11-17.

Research output: Contribution to journalArticle

Butler, Ryan K. ; Ehling, Sarah ; Barbar, Megan ; Thomas, Jess ; Hughes, Mary A. ; Smith, Charles E. ; Pogorelov, Vladimir M. ; Aryal, Dipendra K. ; Wetsel, William C. ; Lascelles, B. Duncan X. / Distinct neuronal populations in the basolateral and central amygdala are activated with acute pain, conditioned fear, and fear-conditioned analgesia. In: Neuroscience Letters. 2017 ; Vol. 661. pp. 11-17.
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AU - Hughes, Mary A.

AU - Smith, Charles E.

AU - Pogorelov, Vladimir M.

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