Distinct ON/OFF fluorescence signals from dual-responsive activatable nanoprobes allows detection of inflammation with improved contrast

Mathieu L. Viger, Guillaume Collet, Jacques Lux, Viet Anh Nguyen Huu, Monica Guma, Alexandra Foucault-Collet, Jason Olejniczak, Shivanjali Joshi-Barr, Gary S. Firestein, Adah Almutairi

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Visualization of biochemical changes associated with disease is of great clinical significance, as it should allow earlier, more accurate diagnosis than structural imaging, facilitating timely clinical intervention. Herein, we report combining stimuli-responsive polymers and near-infrared fluorescent dyes (emission max: 790 nm) to create robust activatable fluorescent nanoprobes capable of simultaneously detecting acidosis and oxidative stress associated with inflammatory microenvironments. The spectrally-resolved mechanism of fluorescence activation allows removal of unwanted background signal (up to 20-fold reduction) and isolation of a pure activated signal, which enables sensitive and unambiguous localization of inflamed areas; target-to-background ratios reach 22 as early as 3 h post-injection. This new detection platform could have significant clinical impact in early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection.

Original languageEnglish (US)
Pages (from-to)119-131
Number of pages13
JournalBiomaterials
Volume133
DOIs
StatePublished - Jul 1 2017

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Keywords

  • Inflammatory microenvironments
  • Molecular imaging
  • NIR optical nanoprobes
  • Spectrally-resolved imaging
  • Stimuli-responsive polymers

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Viger, M. L., Collet, G., Lux, J., Nguyen Huu, V. A., Guma, M., Foucault-Collet, A., Olejniczak, J., Joshi-Barr, S., Firestein, G. S., & Almutairi, A. (2017). Distinct ON/OFF fluorescence signals from dual-responsive activatable nanoprobes allows detection of inflammation with improved contrast. Biomaterials, 133, 119-131. https://doi.org/10.1016/j.biomaterials.2017.03.042