TY - JOUR
T1 - Distinct ON/OFF fluorescence signals from dual-responsive activatable nanoprobes allows detection of inflammation with improved contrast
AU - Viger, Mathieu L.
AU - Collet, Guillaume
AU - Lux, Jacques
AU - Nguyen Huu, Viet Anh
AU - Guma, Monica
AU - Foucault-Collet, Alexandra
AU - Olejniczak, Jason
AU - Joshi-Barr, Shivanjali
AU - Firestein, Gary S.
AU - Almutairi, Adah
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Visualization of biochemical changes associated with disease is of great clinical significance, as it should allow earlier, more accurate diagnosis than structural imaging, facilitating timely clinical intervention. Herein, we report combining stimuli-responsive polymers and near-infrared fluorescent dyes (emission max: 790 nm) to create robust activatable fluorescent nanoprobes capable of simultaneously detecting acidosis and oxidative stress associated with inflammatory microenvironments. The spectrally-resolved mechanism of fluorescence activation allows removal of unwanted background signal (up to 20-fold reduction) and isolation of a pure activated signal, which enables sensitive and unambiguous localization of inflamed areas; target-to-background ratios reach 22 as early as 3 h post-injection. This new detection platform could have significant clinical impact in early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection.
AB - Visualization of biochemical changes associated with disease is of great clinical significance, as it should allow earlier, more accurate diagnosis than structural imaging, facilitating timely clinical intervention. Herein, we report combining stimuli-responsive polymers and near-infrared fluorescent dyes (emission max: 790 nm) to create robust activatable fluorescent nanoprobes capable of simultaneously detecting acidosis and oxidative stress associated with inflammatory microenvironments. The spectrally-resolved mechanism of fluorescence activation allows removal of unwanted background signal (up to 20-fold reduction) and isolation of a pure activated signal, which enables sensitive and unambiguous localization of inflamed areas; target-to-background ratios reach 22 as early as 3 h post-injection. This new detection platform could have significant clinical impact in early detection of pathologies, individual tailoring of drug therapy, and image-guided tumor resection.
KW - Inflammatory microenvironments
KW - Molecular imaging
KW - NIR optical nanoprobes
KW - Spectrally-resolved imaging
KW - Stimuli-responsive polymers
UR - http://www.scopus.com/inward/record.url?scp=85018472989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018472989&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2017.03.042
DO - 10.1016/j.biomaterials.2017.03.042
M3 - Article
C2 - 28433935
AN - SCOPUS:85018472989
SN - 0142-9612
VL - 133
SP - 119
EP - 131
JO - Biomaterials
JF - Biomaterials
ER -