Distinct patterns of chromogranin A-related species can be demonstrated in endocrine cells

Leonard J. Deftos, Adi F. Gazdar, Ruth Hogue-Angeletti, Peter S. Mullen, Douglas W. Burton

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We have studied the pattern of chromogranin A (CgA)-related species in different human endocrine cells that produce CgA and also express the calcitonin gene. Antibodies against CgA peptides that span its linear sequence were used in Western analysis of cell lines derived from medullary thyroid carcinoma (MTC), small cell lung cancers (SCLC), epidermoid cell lung cancer (ECLC) and a pulmonary carcinoid tumor (CRND). Each of the cell lines demonstrated a distinct pattern of CgA-related species. Gel filtration studies also revealed multiple and different forms of immunoreactive CgA in the cell lines. Although proteolysis may contribute to our results, these observations suggest that native CgA is processed to smaller species in a tissue-specific pattern by different endocrine cells. More conclusive studies, however, are necessary to establish that cell processing leads to the specific CgA moieties that we have observed.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalBone and Mineral
Volume9
Issue number3
DOIs
StatePublished - Jun 1990

Keywords

  • Chromogranin A
  • Protein processing

ASJC Scopus subject areas

  • Surgery
  • Biochemistry
  • Endocrinology

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    Deftos, L. J., Gazdar, A. F., Hogue-Angeletti, R., Mullen, P. S., & Burton, D. W. (1990). Distinct patterns of chromogranin A-related species can be demonstrated in endocrine cells. Bone and Mineral, 9(3), 169-178. https://doi.org/10.1016/0169-6009(90)90035-E