Distinct physiologic and neuronal responses to decreased leptin and mild hyperleptinemia

Rexford S. Ahima, Joseph Kelly, Joel K. Elmquist, Jeffrey S. Flier

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Leptin acts on specific populations of hypothalamic neurons to regulate feeding behavior, energy expenditure, and neuroendocrine function. It is not known, however, whether the same neural circuits mediate leptin action across its full biologic dose-response curve, which extends over a broad range, from low levels seen during starvation to high levels characteristic of obesity. Here, we show that the characteristic fall in leptin with fasting causes a rise in neuropeptide Y (NPY) messenger RNA (mRNA), as well as a fall in POMC and cocaine and amphetamine-regulated transcript (CART) mRNAs. Sc infusion of leptin sufficient to maintain plasma levels within the physiologic range during the fast prevents changes in the expression of these peptides, as well as changes in neuroendocrine function, demonstrating that multiple neural circuits are highly sensitive to small changes in leptin within its low physiologic range. In contrast, a modest elevation of plasma leptin above the normal fed range by constant sc infusion, which produced marked reduction in food intake and body weight, decreased NPY mRNA in the arcuate hypothalamic nucleus but did not affect the levels of mRNAs encoding the anorexigenic peptides α-MSH, CART or CRH. These results suggest that the dose response characteristics of leptin on hypothalamic target neurons at the level of mRNA expression are variable, with some neurons (e.g. NPY) responding across a broad dose range and others (e.g. POMC and CART) showing a limited response within the low range. These results further suggest that the central targets of leptin that mediate the transition from starvation to the fed state may be distinct from those that mediate the response to overfeeding and obesity.

Original languageEnglish (US)
Pages (from-to)4923-4931
Number of pages9
JournalEndocrinology
Volume140
Issue number11
StatePublished - 1999

Fingerprint

Leptin
Neuropeptide Y
Amphetamine
Messenger RNA
Cocaine
Pro-Opiomelanocortin
Starvation
Neurons
Obesity
Melanocyte-Stimulating Hormones
Arcuate Nucleus of Hypothalamus
Feeding Behavior
Energy Metabolism
Fasting
Reference Values
Eating
Body Weight
Peptides
Population

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Distinct physiologic and neuronal responses to decreased leptin and mild hyperleptinemia. / Ahima, Rexford S.; Kelly, Joseph; Elmquist, Joel K.; Flier, Jeffrey S.

In: Endocrinology, Vol. 140, No. 11, 1999, p. 4923-4931.

Research output: Contribution to journalArticle

Ahima, Rexford S. ; Kelly, Joseph ; Elmquist, Joel K. ; Flier, Jeffrey S. / Distinct physiologic and neuronal responses to decreased leptin and mild hyperleptinemia. In: Endocrinology. 1999 ; Vol. 140, No. 11. pp. 4923-4931.
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