TY - JOUR
T1 - Distinct vascular and intestinal smooth muscle myosin heavy chain mRNAs are encoded by a single-copy gene in the chicken
AU - Hamada, Yoshio
AU - Yanagisawa, Masashi
AU - Katsuragawa, Yoshinari
AU - Coleman, John R.
AU - Nagata, Shuichi
AU - Matsuda, Genji
AU - Masaki, Tomoh
N1 - Funding Information:
We thank Dr. T.S. Okada for continuous encouragement. This work was supported in pm by research grants from the Ministry of Education, Science and Culture, Japan (T.M.), the Uehara Memorial Foundation (Y.H.), and the National Science Foundation (J.C.).
PY - 1990/7/16
Y1 - 1990/7/16
N2 - We examined whether the gizzard MHC gene is expressed in other smooth muscle tissues and, if so, whether there exist any smooth muscle MHC isoforms at the mRNA level. Northern blot analysis showed that the gizzard MHC gene was also expressed in the aorta and jejunum, but not in the pectoralis muscle or in fibroblasts. This indicates that striated muscle and non-muscle MHC isoforms are encoded in genes distinct from the smooth muscle MHC gene. Further, nuclease S1 mapping showed that the aortic smooth muscle MHC mRNA was distinct from the gizzard mRNA in the 5′-terminal coding region. Both of these mRNA species are expressed in the jejunum. These observations suggest that there exist at least two chicken smooth muscle MHC isoforms, vascular-type and intestinal-type, and that these isoforms are generated from a single-copy gene, probably by an alternative mRNA processing mechanism.
AB - We examined whether the gizzard MHC gene is expressed in other smooth muscle tissues and, if so, whether there exist any smooth muscle MHC isoforms at the mRNA level. Northern blot analysis showed that the gizzard MHC gene was also expressed in the aorta and jejunum, but not in the pectoralis muscle or in fibroblasts. This indicates that striated muscle and non-muscle MHC isoforms are encoded in genes distinct from the smooth muscle MHC gene. Further, nuclease S1 mapping showed that the aortic smooth muscle MHC mRNA was distinct from the gizzard mRNA in the 5′-terminal coding region. Both of these mRNA species are expressed in the jejunum. These observations suggest that there exist at least two chicken smooth muscle MHC isoforms, vascular-type and intestinal-type, and that these isoforms are generated from a single-copy gene, probably by an alternative mRNA processing mechanism.
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U2 - 10.1016/0006-291X(90)91239-O
DO - 10.1016/0006-291X(90)91239-O
M3 - Article
C2 - 2372298
AN - SCOPUS:0025120556
SN - 0006-291X
VL - 170
SP - 53
EP - 58
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -