Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis

Seul Ji Lee, Young Na Yum, Sang Cheol Kim, Yuneung Kim, Johan Lim, Won Jun Lee, Kyung Hye Koo, Joo Hwan Kim, Jee Eun Kim, Woo Sun Lee, Soojung Sohn, Sue Nie Park, Jeong Hill Park, Jeongmi Lee, Sung Won Kwon

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A rapid and sensitive method to determine the characteristics of carcinogens is needed. In this study, we used a microarray-based genomics approach, with a short-term in vivo model, in combination with insights from statistical and mechanistic analyses to determine the characteristics of carcinogens. Carcinogens were evaluated based on the different mechanisms involved in the responses to genotoxic carcinogens and non-genotoxic carcinogens. Gene profiling was performed at two time points after treatment with six training and four test carcinogens. We mapped the DEG (differentially expressed gene)-related pathways to analyze cellular processes, and we discovered significant mechanisms that involve critical cellular components. Classification results were further supported by Comet and Micronucleus assays. Mechanistic studies based on gene expression profiling enhanced our understanding of the characteristics of different carcinogens. Moreover, the efficiency of this study was demonstrated by the short-term nature of the animal experiments that were conducted.

Original languageEnglish (US)
Article number2783
JournalScientific reports
Volume3
DOIs
StatePublished - Oct 14 2013

ASJC Scopus subject areas

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    Lee, S. J., Yum, Y. N., Kim, S. C., Kim, Y., Lim, J., Lee, W. J., Koo, K. H., Kim, J. H., Kim, J. E., Lee, W. S., Sohn, S., Park, S. N., Park, J. H., Lee, J., & Kwon, S. W. (2013). Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis. Scientific reports, 3, [2783]. https://doi.org/10.1038/srep02783