Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices

Hongmei Jiang, Sangmyung Rhee, Chin Han Ho, Frederick Grinnell

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Understanding growth factor function during wound repair is necessary for the development of therapeutic interventions to improve healing outcomes. In the current study, we compare the effects of serum and purified growth factors on human fibroblast function in three different collagen matrix models: cell migration in nested matrices, floating matrix contraction, and stressed-released matrix contraction. The results of these studies indicate that platelet-derived growth factor (PDGF) is unique in its capacity to promote cell migration. Serum, lysophosphatidic acid, sphingosine-1-phophate (S1P), and endothelin-1 promote stressed-released matrix contraction but not cell migration. In addition, we found that S1P inhibits fibroblast migration and treatment of serum to remove lipid growth factors or treatment of cells to interfere with S1P2 receptor function increases serum promigratory activity. Our findings suggest that different sets of growth factors generate promigratory and procontractile tissue environments for fibroblasts and that the balance between PDGF and S1P is a key determinant of fibroblast promigratory activity.

Original languageEnglish (US)
Pages (from-to)2151-2160
Number of pages10
JournalFASEB Journal
Volume22
Issue number7
DOIs
StatePublished - Jul 2008

Fingerprint

Fibroblasts
sphingosine
growth factors
Sphingosine
fibroblasts
collagen
Intercellular Signaling Peptides and Proteins
Collagen
cell movement
Cell Movement
platelet-derived growth factor
Platelet-Derived Growth Factor
Serum
Lysosphingolipid Receptors
endothelins
Endothelin-1
animal injuries
Lipids
Repair
therapeutics

Keywords

  • Cell contraction
  • Cell migration
  • Lysophosphatidic acid
  • Platelet-derived growth factor
  • Sphingosine-1-phosphate
  • Wound repair

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices. / Jiang, Hongmei; Rhee, Sangmyung; Ho, Chin Han; Grinnell, Frederick.

In: FASEB Journal, Vol. 22, No. 7, 07.2008, p. 2151-2160.

Research output: Contribution to journalArticle

Jiang, Hongmei ; Rhee, Sangmyung ; Ho, Chin Han ; Grinnell, Frederick. / Distinguishing fibroblast promigratory and procontractile growth factor environments in 3-D collagen matrices. In: FASEB Journal. 2008 ; Vol. 22, No. 7. pp. 2151-2160.
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