Distribution and concentration of TRH in the rat brain

C. Oliver, R. L. Eskay, N. Ben Jonathan, J. C. Porter

Research output: Contribution to journalArticle

264 Citations (Scopus)

Abstract

Qualitative and quantitative criteria indicate that thyrotropin releasing hormone (TRH) is present in methanolic extracts prepared from all regions of the rat brain. The conclusion that TRH in the extracts of rat brain is the same as synthetic TRH is based on the following criteria: immunoreactivity with a specific antiserum to synthetic TRH; chromatographic properties on Sephadex G-10; electrophoretic mobility on cellulose acetate; susceptibility to degradation by plasma; and capacity of the extracts to stimulate thyrotropin (TSH) release in vitro from anterior pituitary tissue. The greatest concentration of TRH, determined by radioimmunoassay, was found in the hypothalamus, followed by the posterior pituitary gland, thalamus, brain stem, cerebrum, anterior pituitary gland, and cerebellum. The total quantity of TRH in the rat brain was 18.2 ng. Of this amount, 33.6% was present in the thalamus; 26.7% in the cerebrum; 18.8% in the hypothalamus; 18.6% in the brain stem; 1.6% in the cerebellum; 0.42% in the posterior pituitary gland; and 0.09% in the anterior pituitary gland. On the basis of these findings, it is concluded that significant quantities of TRH are present in the extrahypothalamic regions of the brain as well as in the hypothalamic regions. (26 references)

Original languageEnglish (US)
Pages (from-to)540-546
Number of pages7
JournalEndocrinology
Volume95
Issue number2
StatePublished - 1974

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Thyrotropin-Releasing Hormone
Brain
Posterior Pituitary Gland
Anterior Pituitary Gland
Cerebrum
Thalamus
Cerebellum
Hypothalamus
Brain Stem
Thyrotropin
Radioimmunoassay
Immune Sera

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Oliver, C., Eskay, R. L., Ben Jonathan, N., & Porter, J. C. (1974). Distribution and concentration of TRH in the rat brain. Endocrinology, 95(2), 540-546.

Distribution and concentration of TRH in the rat brain. / Oliver, C.; Eskay, R. L.; Ben Jonathan, N.; Porter, J. C.

In: Endocrinology, Vol. 95, No. 2, 1974, p. 540-546.

Research output: Contribution to journalArticle

Oliver, C, Eskay, RL, Ben Jonathan, N & Porter, JC 1974, 'Distribution and concentration of TRH in the rat brain', Endocrinology, vol. 95, no. 2, pp. 540-546.
Oliver C, Eskay RL, Ben Jonathan N, Porter JC. Distribution and concentration of TRH in the rat brain. Endocrinology. 1974;95(2):540-546.
Oliver, C. ; Eskay, R. L. ; Ben Jonathan, N. ; Porter, J. C. / Distribution and concentration of TRH in the rat brain. In: Endocrinology. 1974 ; Vol. 95, No. 2. pp. 540-546.
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