Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus

B. G. Irani, J. Donato, D. P. Olson, B. B. Lowell, T. C. Sacktor, M. E. Reyland, K. P. Tolson, A. R. Zinn, Y. Ueta, I. Sakata, J. M. Zigman, C. F. Elias, D. J. Clegg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

PKC-theta (PKC-θ), a member of the novel protein kinase C family (nPKC), regulates a wide variety of functions in the periphery. However, its presence and role in the CNS has remained largely unknown. Recently, we demonstrated the presence of PKC-θ in the arcuate hypothalamic nucleus (ARC) and knockdown of PKC-θ from the ARC protected mice from developing diet-induced obesity. Another isoform of the nPKC group, PKC-delta (PKC-δ), is expressed in several non-hypothalamic brain sites including the thalamus and hippocampus. Although PKC-δ has been implicated in regulating hypothalamic glucose homeostasis, its distribution in the hypothalamus has not previously been described. In the current study, we used immunohistochemistry to examine the distribution of PKC-θ and -δ immunoreactivity in rat and mouse hypothalamus. We found PKC-θ immunoreactive neurons in several hypothalamic nuclei including the ARC, lateral hypothalamic area, perifornical area and tuberomammillary nucleus. PKC-δ immunoreactive neurons were found in the paraventricular and supraoptic nuclei. Double-label immunohistochemisty in mice expressing green fluorescent protein either with the long form of leptin receptor (LepR-b) or in orexin (ORX) neurons indicated that PKC-θ is highly colocalized in lateral hypothalamic ORX neurons but not in lateral hypothalamic LepR-b neurons. Double-label immunohistochemistry in oxytocin-enhanced yellow fluorescent protein mice or arginine vasopressin-enhanced green fluorescent protein (AVP-EGFP) transgenic rats revealed a high degree of colocalization of PKC-δ within paraventricular and supraoptic oxytocin neurons but not the vasopressinergic neurons. We conclude that PKC-θ and -δ are expressed in different hypothalamic neuronal populations.

Original languageEnglish (US)
Pages (from-to)1065-1079
Number of pages15
JournalNeuroscience
Volume170
Issue number4
DOIs
StatePublished - Nov 2010

Fingerprint

Protein Kinase C-delta
Hypothalamus
Rodentia
Neurons
AIDS-Related Complex
Lateral Hypothalamic Area
Oxytocin
Protein Kinase C
Immunohistochemistry
Transgenic Rats
Leptin Receptors
Supraoptic Nucleus
Arcuate Nucleus of Hypothalamus
Arginine Vasopressin
Paraventricular Hypothalamic Nucleus
Green Fluorescent Proteins
Thalamus
Hippocampus
Protein Isoforms
Homeostasis

Keywords

  • Arousal
  • Feeding
  • Glutamate
  • Lateral hypothalamic area
  • Orexin
  • Oxytocin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Irani, B. G., Donato, J., Olson, D. P., Lowell, B. B., Sacktor, T. C., Reyland, M. E., ... Clegg, D. J. (2010). Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus. Neuroscience, 170(4), 1065-1079. https://doi.org/10.1016/j.neuroscience.2010.07.064

Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus. / Irani, B. G.; Donato, J.; Olson, D. P.; Lowell, B. B.; Sacktor, T. C.; Reyland, M. E.; Tolson, K. P.; Zinn, A. R.; Ueta, Y.; Sakata, I.; Zigman, J. M.; Elias, C. F.; Clegg, D. J.

In: Neuroscience, Vol. 170, No. 4, 11.2010, p. 1065-1079.

Research output: Contribution to journalArticle

Irani, BG, Donato, J, Olson, DP, Lowell, BB, Sacktor, TC, Reyland, ME, Tolson, KP, Zinn, AR, Ueta, Y, Sakata, I, Zigman, JM, Elias, CF & Clegg, DJ 2010, 'Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus', Neuroscience, vol. 170, no. 4, pp. 1065-1079. https://doi.org/10.1016/j.neuroscience.2010.07.064
Irani, B. G. ; Donato, J. ; Olson, D. P. ; Lowell, B. B. ; Sacktor, T. C. ; Reyland, M. E. ; Tolson, K. P. ; Zinn, A. R. ; Ueta, Y. ; Sakata, I. ; Zigman, J. M. ; Elias, C. F. ; Clegg, D. J. / Distribution and neurochemical characterization of protein kinase C-theta and -delta in the rodent hypothalamus. In: Neuroscience. 2010 ; Vol. 170, No. 4. pp. 1065-1079.
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