TY - JOUR
T1 - Distribution of exogenously administered renin in mouse kidney
AU - Iwao, Hiroshi
AU - Nakamura, Norifumi
AU - Ikemoto, Fumihiko
AU - Yamamoto, Kenjiro
AU - Mizuhira, Vinci
AU - Ono, Michiko
AU - Sugiura, Yoshie
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research 56440029, a Grant-in-Aid for Cooperative Research 56370009, a Grant-in-Aid for Encouragement of Young Scientists 56770133 from the Ministry of Education, Science and Culture, and a Research Grant for Cardiovascular Diseases(54-A2) from the Ministry of Health and Welfare. We are grateful to M.Ohara for assistance with the manuscript.
PY - 1982
Y1 - 1982
N2 - Purified mouse submaxillary gland renin was labelled with iodine (125I) by the chloramine-T method and this 125I labelled renin was given to male ICR mice(6-7 wks. old), intravenously in a dose of 20 ng(2 μCi)/40 g body weight. At a specified time, the kidneys of these treated mice were excised, the radioactivity determined and the tissues immediately prepared for microscope and electron microscope autoradiography(micro-ARG and EM-ARG). Silver grains accumulated mainly at the apical site of the proximal convoluted tubules but were not identified in other areas of the nephron, including macula densa or in the arterial wall, glomeruli, and juxtaglomerular cells(micro-ARG). Silver grains were seen in the pinocytotic vesicles, vacuoles and lysosomal granules(EM-ARG). This is the first demonstration that exogenous renin is ultrafiltered through glomerular capillaries and is reabsorbed by pinocytosis in the one-third upper area of the proximal convoluted tubule.
AB - Purified mouse submaxillary gland renin was labelled with iodine (125I) by the chloramine-T method and this 125I labelled renin was given to male ICR mice(6-7 wks. old), intravenously in a dose of 20 ng(2 μCi)/40 g body weight. At a specified time, the kidneys of these treated mice were excised, the radioactivity determined and the tissues immediately prepared for microscope and electron microscope autoradiography(micro-ARG and EM-ARG). Silver grains accumulated mainly at the apical site of the proximal convoluted tubules but were not identified in other areas of the nephron, including macula densa or in the arterial wall, glomeruli, and juxtaglomerular cells(micro-ARG). Silver grains were seen in the pinocytotic vesicles, vacuoles and lysosomal granules(EM-ARG). This is the first demonstration that exogenous renin is ultrafiltered through glomerular capillaries and is reabsorbed by pinocytosis in the one-third upper area of the proximal convoluted tubule.
UR - http://www.scopus.com/inward/record.url?scp=0020444156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020444156&partnerID=8YFLogxK
U2 - 10.3109/10641968209062402
DO - 10.3109/10641968209062402
M3 - Article
C2 - 6756708
AN - SCOPUS:0020444156
VL - A4
SP - 2449
EP - 2456
JO - Clinical and Experimental Hypertension
JF - Clinical and Experimental Hypertension
SN - 1064-1963
IS - 11-12
ER -