Disulfide trapping the mechanosensitive channel MscL into a gating-transition state

Irene Iscla, Gal Levin, Robin Wray, Paul Blount

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The mechanosensitive channel of large conductance, MscL, serves as a biological emergency release valve protecting bacteria from acute osmotic downshock, and is to date the best characterized mechanosensitive channel. The N-terminal region of the protein has been shown to be critical for function by random, site-directed, and deletion mutagenesis, yet is structurally poorly understood. One model proposes that the extreme N-termini form a cluster of amphipathic helices that serves as a cytoplasmic second gate, separated from the pore-forming transmembrane domain by a "linker". Here, we have utilized cysteine trapping of single-cysteine mutated channels to determine the proximity, within the homopentameric complex, of residues within and just peripheral to this proposed linker. Our results indicate that all residues in this region can form disulfide bridges, and that the percentage of dimers increases when the channel is gated in vivo. Functional studies suggest that oxidation traps one of these mutated channels, N15C, into a gating-transition state that retains the capacity to obtain both fully open and closed states. The data are not easily explained by current models for the smooth transition from closed-to-open states, but predict that an asymmetric movement of one or more of the subunits commonly occurs upon gating.

Original languageEnglish (US)
Pages (from-to)1224-1232
Number of pages9
JournalBiophysical Journal
Volume92
Issue number4
DOIs
StatePublished - Feb 2007

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Disulfides
Cysteine
Site-Directed Mutagenesis
Emergencies
Bacteria
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Disulfide trapping the mechanosensitive channel MscL into a gating-transition state. / Iscla, Irene; Levin, Gal; Wray, Robin; Blount, Paul.

In: Biophysical Journal, Vol. 92, No. 4, 02.2007, p. 1224-1232.

Research output: Contribution to journalArticle

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