Diversity of the G-protein family

Sequences from five additional α subunits in the mouse

M. Strathmann, T. M. Wilkie, M. I. Simon

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Biochemical analysis has revealed a number of guanine nucleotide-binding regulatory proteins (G proteins) that mediate signal transduction in mammalian systems. Characterization of their cDNAs uncovered a family of proteins with regions of highly conserved amino acid sequence. To examine the extent of diversity of the G protein family, we used the polymerase chain reaction to detect additional gene products in mouse brain and spermatid RNA that share these conserved regions. Sequences corresponding to six of the eight known G protein α subunits were obtained. In addition, we found sequences corresponding to five newly discovered α subunits. Our results suggest that the complexity of the G protein family is much greater than previously suspected.

Original languageEnglish (US)
Pages (from-to)7407-7409
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number19
StatePublished - 1989

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GTP-Binding Proteins
Carrier Proteins
Spermatids
Protein Subunits
Amino Acid Sequence
Signal Transduction
Complementary DNA
RNA
Polymerase Chain Reaction
Brain
Genes
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Diversity of the G-protein family : Sequences from five additional α subunits in the mouse. / Strathmann, M.; Wilkie, T. M.; Simon, M. I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 19, 1989, p. 7407-7409.

Research output: Contribution to journalArticle

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