TY - JOUR
T1 - DNA analysis at p53 locus in carcinomas arising from pleomorphic adenomas of salivary glands
T2 - Comparison of molecular study and p53 immunostaining
AU - Yamamoto, Yuzo
AU - Kishimoto, Yosuke
AU - Wistuba, Ignacio I.
AU - Virmani, Arvind K.
AU - Vuitch, Frank
AU - Gazdar, Adi F.
AU - Albores-Saavedra, Jorge
PY - 1998
Y1 - 1998
N2 - Where and how frequently p53 abnormalities are involved in the development of pleomorphic adenoma (PA) and its malignant progression to carcinoma was investigated. The presence of p53 gene abnormalities was analyzed in eight patients with carcinoma in pleomorphic adenoma (CPA) by polymerase chain reaction (PCR)-based assays and immunohistochemistry. Normal salivary gland tissue, adenomatous, transitional and carcinomatous areas were microdissected from archival microslides and analyzed for allelic deletions of the p53 gene using two microsatellite markers at the p53 locus; dinucleotide (CA)(n) repeat and pentanucleotide (AAAAT)(n) repeat. Loss of heterozygosity (LOH) of the p53 gene was detected in 57% of adenomas, 86% of transitional lesions and 86% of carcinomas. In contrast, overexpression of p53 oncoprotein was noted immunohistochemically in 13% of adenomas, 50% of transitional areas and 75% of carcinomas. All of the tumors with immunoreactivity for p53 oncoprotein demonstrated LOH. Moreover, when LOH was present in adenomatous or transitional areas, the identical LOH was always detected in the corresponding carcinomatous areas in the same CPA tumors. These findings indicate that p53gene mutation is an early event and occurs frequently at an early stage of precancerous lesions and may be responsible for most cases of malignant transformation of PA.
AB - Where and how frequently p53 abnormalities are involved in the development of pleomorphic adenoma (PA) and its malignant progression to carcinoma was investigated. The presence of p53 gene abnormalities was analyzed in eight patients with carcinoma in pleomorphic adenoma (CPA) by polymerase chain reaction (PCR)-based assays and immunohistochemistry. Normal salivary gland tissue, adenomatous, transitional and carcinomatous areas were microdissected from archival microslides and analyzed for allelic deletions of the p53 gene using two microsatellite markers at the p53 locus; dinucleotide (CA)(n) repeat and pentanucleotide (AAAAT)(n) repeat. Loss of heterozygosity (LOH) of the p53 gene was detected in 57% of adenomas, 86% of transitional lesions and 86% of carcinomas. In contrast, overexpression of p53 oncoprotein was noted immunohistochemically in 13% of adenomas, 50% of transitional areas and 75% of carcinomas. All of the tumors with immunoreactivity for p53 oncoprotein demonstrated LOH. Moreover, when LOH was present in adenomatous or transitional areas, the identical LOH was always detected in the corresponding carcinomatous areas in the same CPA tumors. These findings indicate that p53gene mutation is an early event and occurs frequently at an early stage of precancerous lesions and may be responsible for most cases of malignant transformation of PA.
KW - Immunohistochemistry
KW - Loss of heterozygosity
KW - P53 gene
KW - Pleomorphic adenoma
KW - Salivary gland carcinoma
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U2 - 10.1111/j.1440-1827.1998.tb03904.x
DO - 10.1111/j.1440-1827.1998.tb03904.x
M3 - Article
C2 - 9648154
AN - SCOPUS:0031818208
SN - 1320-5463
VL - 48
SP - 265
EP - 272
JO - Pathology International
JF - Pathology International
IS - 4
ER -