DNA analysis at p53 locus in carcinomas arising from pleomorphic adenomas of salivary glands: Comparison of molecular study and p53 immunostaining

Yuzo Yamamoto, Yosuke Kishimoto, Ignacio I. Wistuba, Arvind K. Virmani, Frank Vuitch, Adi F. Gazdar, Jorge Albores-Saavedra

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Where and how frequently p53 abnormalities are involved in the development of pleomorphic adenoma (PA) and its malignant progression to carcinoma was investigated. The presence of p53 gene abnormalities was analyzed in eight patients with carcinoma in pleomorphic adenoma (CPA) by polymerase chain reaction (PCR)-based assays and immunohistochemistry. Normal salivary gland tissue, adenomatous, transitional and carcinomatous areas were microdissected from archival microslides and analyzed for allelic deletions of the p53 gene using two microsatellite markers at the p53 locus; dinucleotide (CA)(n) repeat and pentanucleotide (AAAAT)(n) repeat. Loss of heterozygosity (LOH) of the p53 gene was detected in 57% of adenomas, 86% of transitional lesions and 86% of carcinomas. In contrast, overexpression of p53 oncoprotein was noted immunohistochemically in 13% of adenomas, 50% of transitional areas and 75% of carcinomas. All of the tumors with immunoreactivity for p53 oncoprotein demonstrated LOH. Moreover, when LOH was present in adenomatous or transitional areas, the identical LOH was always detected in the corresponding carcinomatous areas in the same CPA tumors. These findings indicate that p53gene mutation is an early event and occurs frequently at an early stage of precancerous lesions and may be responsible for most cases of malignant transformation of PA.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalPathology International
Volume48
Issue number4
DOIs
StatePublished - Jan 1 1998

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Keywords

  • Immunohistochemistry
  • Loss of heterozygosity
  • P53 gene
  • Pleomorphic adenoma
  • Salivary gland carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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