DNA binding is essential for PprI function in response to radiation damage in Deinococcus radiodurans

Huiming Lu, Huan Chen, Guangzhi Xu, Amir Miraj Ul Hussain Shah, Yuejin Hua

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The extremely radioresistant bacterium. Deinococcus radiodurans possesses a rapid and efficient but poorly known DNA damage response mechanism that mobilizes one-third of its genome to survive lethal radiation damage. Deinococcal PprI serves as a general switch to regulate the expression of dozens of proteins from different pathways after radiation, including the DNA repair proteins RecA, PprA and SSB. However, the underlying mechanism is poorly understood. In this study, we analyzed the dynamic alteration in global transcriptional profiles in wildtype and. pprI mutant strains by combining microarrays and time-course sampling. We found that PprI up-regulated transcription of at least 210 genes after radiation, including 21 DNA repair and replication-related genes. We purified PprI and a helix-turn-helix (HTH) domain mutant and found that PprI specifically bound to the promoters of. recA and. pprA in vitro but did not bind nonspecific double-strand DNA. Chromatin immunoprecipitation (ChIP) assays confirmed that PprI specifically interacted with the promoter DNA of. recA and. pprA after radiation. Finally, we showed that a DNA-binding activity-deficient pprI mutant only partially restored resistance of the. pprI mutant strain to γ radiation, UV radiation, and mitomycin C. Taken together, these results indicate that DNA-binding activity is essential for PprI to program the DNA repair process and cellular survival of. D. radiodurans in response to radiation damage.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalDNA Repair
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Deinococcus
Radiation damage
Radiation
DNA
DNA Repair
Repair
Genes
Rec A Recombinases
Chromatin Immunoprecipitation
Mitomycin
DNA Replication
Transcription
Microarrays
DNA Damage
Ultraviolet radiation
Chromatin
Assays
Bacteria
Genome
Switches

Keywords

  • Deinococcus radiodurans
  • DNA damage
  • Ionizing radiation
  • PprI
  • Response regulator

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

DNA binding is essential for PprI function in response to radiation damage in Deinococcus radiodurans. / Lu, Huiming; Chen, Huan; Xu, Guangzhi; Shah, Amir Miraj Ul Hussain; Hua, Yuejin.

In: DNA Repair, Vol. 11, No. 2, 01.02.2012, p. 139-145.

Research output: Contribution to journalArticle

Lu, Huiming ; Chen, Huan ; Xu, Guangzhi ; Shah, Amir Miraj Ul Hussain ; Hua, Yuejin. / DNA binding is essential for PprI function in response to radiation damage in Deinococcus radiodurans. In: DNA Repair. 2012 ; Vol. 11, No. 2. pp. 139-145.
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