DNA-damage processing in a radiation-sensitive mouse cell line

Raymond L. Warters, Bradley W. Lyons, David J. Chen, Koki Sato

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalMutation Research-DNA Repair
Volume293
Issue number2
DOIs
StatePublished - Jan 1993

Keywords

  • DNA double-strand break repair mutants
  • DNA-damage processing
  • Mouse L cells

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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