DNA-damage processing in a radiation-sensitive mouse cell line

Raymond L. Warters, Bradley W. Lyons, David J. Chen, Koki Sato

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalMutation Research-DNA Repair
Volume293
Issue number2
DOIs
StatePublished - 1993

Fingerprint

DNA Damage
Cells
Radiation
Cell Line
DNA
Processing
Double-Stranded DNA Breaks
Repair
Single-Stranded DNA Breaks
Radiation Tolerance
Defects

Keywords

  • DNA double-strand break repair mutants
  • DNA-damage processing
  • Mouse L cells

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Toxicology

Cite this

DNA-damage processing in a radiation-sensitive mouse cell line. / Warters, Raymond L.; Lyons, Bradley W.; Chen, David J.; Sato, Koki.

In: Mutation Research-DNA Repair, Vol. 293, No. 2, 1993, p. 91-98.

Research output: Contribution to journalArticle

Warters, Raymond L. ; Lyons, Bradley W. ; Chen, David J. ; Sato, Koki. / DNA-damage processing in a radiation-sensitive mouse cell line. In: Mutation Research-DNA Repair. 1993 ; Vol. 293, No. 2. pp. 91-98.
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