Abstract
The induction and repair of radiation-induced DNA damage was assessed in 3 mouse cell lines, including the parental L cell line, a radiation-sensitive, SL3-147 mutant cell line and the H5 revertant to radiation resistance. The yield of neither radiation-induced DNA single- nor double-strand breaks could explain the variable sensitivity of the 3 cell lines. Closure of DNA single-strand breaks proceeded at a similar rate in both the L and SL3-147 cell lines. Closure of DNA double-strand breaks however was significantly slower and less complete in the SL3-147 cell line than in either of the radiation-resistant cell lines. The results are consistent with the increased radiation sensitivity of the SL3-147 cell line resulting from a defect in their ability to repair radiation-induced DNA double-strand breaks.
Original language | English (US) |
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Pages (from-to) | 91-98 |
Number of pages | 8 |
Journal | Mutation Research-DNA Repair |
Volume | 293 |
Issue number | 2 |
DOIs | |
State | Published - Jan 1993 |
Keywords
- DNA double-strand break repair mutants
- DNA-damage processing
- Mouse L cells
ASJC Scopus subject areas
- Molecular Biology
- Toxicology
- Genetics