DNA-dependent protein kinase does not play a role in adaptive survival responses to ionizing radiation

Eric Odegaard, Chin Rang Yang, David A. Boothman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We previously demonstrated that exposure of certain human tumor cells to very low chronic doses of ionizing radiation led to their enhanced survival following exposure to subsequent high doses of radiation. Survival enhancement due to these adaptive survival responses (ASRs) ranged from 1.5-fold to 2.2-fold in many human tumor cells. Furthermore, we showed that ASRs result from altered G1 checkpoint regulation, possibly mediated by overexpression of cyclin D1, proliferating cell nuclear antigen (PCNA), and the X-ray induction of cyclin A. Because cyclin D1 and PCNA proteins are components of many DNA synthetic and repair processes in the cell, we tested the hypothesis that preexposure of cells to low doses of ionizing radiation enabled activation of the DNA repair machinery needed for survival recovery after high-dose radiation. We examined the role of DNA break repair in ASRs using murine cells deficient (i.e., severe combined immunodeficiency [SCID] cells) or proficient (i.e., parental mouse strain [CB-17] cells) in DNA-dependent protein kinase catalytic subunit (DNA-PKcs) expression and DNA double-strand break repair. DNA-PKcs is a nuclear serine/threonine protein kinase that is activated by DNA breaks and plays a key role in double-strand break repair. DNA-PKcs also phosphorylates several nuclear DNA-binding regulatory transcription factor proteins (e.g., Sp1 and p53), which suggests that DNA-PKcs may play a role in regulating transcription, replication, and recombination as well as DNA repair, after radiation. Therefore, we exposed confluent SCID or CB-17 cells to low priming doses of ionizing radiation (i.e., 5 cGy) and compared the survival responses of primed cells to those of unprimed cells after an equitoxic high-dose challenge. Low-dose-primed SCID or CB-17 cells demonstrated 2-fold enhanced survival after a high-dose challenge compared to that of unprimed control cells. These data suggest that expression of the catalytic subunit of DNA-PKcs (expressed in CB-17 not SCID cells) and the presence of active double-strand break repair processes (active in CB-17, deficient in SCID cells) do not play a major role in ASRs in mammalian cells. Furthermore, we present data that suggest that DNA-PKcs plays a role in the regulation of the G2/M cell cycle checkpoint following extremely high doses of ionizing radiation.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalEnvironmental Health Perspectives
Volume106
Issue numberSUPPL. 1
StatePublished - 1998

Fingerprint

DNA-Activated Protein Kinase
Ionizing radiation
Ionizing Radiation
Repair
Dosimetry
DNA
protein
Survival
repair
Severe Combined Immunodeficiency
Cells
Catalytic Domain
Cyclin D1
Proliferating Cell Nuclear Antigen
DNA Repair
Tumors
Catalytic DNA
Radiation
Cyclin A
Protein-Serine-Threonine Kinases

Keywords

  • Adaptive survival responses
  • DNA double-strand break repair
  • DNA-dependent protein kinase
  • SCID mice
  • X rays

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

DNA-dependent protein kinase does not play a role in adaptive survival responses to ionizing radiation. / Odegaard, Eric; Yang, Chin Rang; Boothman, David A.

In: Environmental Health Perspectives, Vol. 106, No. SUPPL. 1, 1998, p. 301-305.

Research output: Contribution to journalArticle

Odegaard, Eric ; Yang, Chin Rang ; Boothman, David A. / DNA-dependent protein kinase does not play a role in adaptive survival responses to ionizing radiation. In: Environmental Health Perspectives. 1998 ; Vol. 106, No. SUPPL. 1. pp. 301-305.
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