DNA double-strand break repair in two radiation-sensitive mouse mammary carcinoma cell lines

Raymond L. Warters, Louis R. Barrows, David J. Chen

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The capacity of two radiation-sensitive clones (SX9 and SX10) of the mouse mammary carcinoma cell line SR1 to rejoin radiation-induced DNA double-strand breaks (DSBs) was determined by pulsed-field agarose gel electrophoresis. DSBs were produced with equivalent efficiency in all three cell lines. Both the SX9 and SX10 cell lines demonstrated a significantly diminished capacity to rejoin radiation-induced DSBs. The fraction of the original DNA DSB damage remaining in the DNA of 20 Gy-exposed SR1, SX9 and SX10 cells after 6 h of 37°C incubation was estimated to be 14, 82 and 54%, respectively. In addition the SX10 cell line exhibited enhanced cytotoxicity when exposed to the DNA topoisomerase II poison mitoxantrone. The results indicate that both the SX9 and SX10 cell lines are DNA DSB repair mutants.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalMutation Research-DNA Repair
Volume336
Issue number1
DOIs
StatePublished - Jan 1995

Keywords

  • DNA double-strand break repair
  • Mouse mammary carcinoma
  • X-irradiation

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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