DNA methylation affects cell proliferation, cortisol secretion and steroidogenic gene expression in human adrenocortical NCI-H295R cells

Jiangi Liu, X. D. Li, A. Vaheri, R. Voutilainen

Research output: Contribution to journalArticle

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Abstract

Aberrant DNA methylation may be involved in human adrenocortical tumorigenesis, which is often accompanied by abnormal hormone production. In this study, we aimed to clarify the effects of DNA methylation on steroidogenesis using the human adrenocortical NCI-H295R cell line as a model. Treatment with the DNA methylation inhibitor 5-aza-2′-deoxycytidine (Azad; 10 μM for 7 days) decreased the proliferation rate to approximately 20% and the cell number to 60% of the control, with a simultaneous increase in the expression of the cyclin-dependent kinase inhibitor p57kip2 gene. In addition, Azad treatment increased cortisol secretion dose and time dependently, whereas dehydroepiandrosterone sulfate secretion was not affected. Azad treatment decreased basal and (BU)2cAMP-induced expression of low- and high-density lipoprotein receptor, steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme, steroid 17α-hydroxylase/17,20-lyase and steroid 21-hydroxylase mRNA, as well as the StAR protein level. In contrast, Azad treatment increased the basal expression of steroid 11β-hydroxylase and 3β-hydroxysteroid dehydrogenase/Δ54-isomerase genes, although it inhibited the (Bu 2cAMP-induced expression of these two genes. The expression of steroidogenic factor-1 (SF-1) and DAX-1 (dosage-sensitive sex reversal-adrenal hypoplasia congenita critical region on the X-chromosome 1) genes (both harboring putative CpG islands in their promoters) and the methylation degree of the HpaII recognition site(s) in the SF-1 gene promoter region were reduced by Azad treatment. The immunostaining pattern of the methyl-CpG-binding protein MeCP2 was also modified by Azad treatment. These results suggest that DNA methylation may be implicated in the regulation of cell proliferation and steroidogenesis in human adrenocortical cells.

Original languageEnglish (US)
Pages (from-to)651-662
Number of pages12
JournalJournal of Molecular Endocrinology
Volume33
Issue number3
DOIs
StatePublished - Dec 2004

Fingerprint

DNA Methylation
Hydrocortisone
Steroidogenic Factor 1
Cell Proliferation
Steroid 17-alpha-Hydroxylase
Gene Expression
decitabine
3-Hydroxysteroid Dehydrogenases
Steroid 11-beta-Hydroxylase
Cholesterol Side-Chain Cleavage Enzyme
Genes
Steroid 21-Hydroxylase
Isomerases
X-Linked Genes
Dehydroepiandrosterone Sulfate
CpG Islands
Cyclin-Dependent Kinases
Chromosomes, Human, Pair 1
Genetic Promoter Regions
Methylation

ASJC Scopus subject areas

  • Endocrinology

Cite this

DNA methylation affects cell proliferation, cortisol secretion and steroidogenic gene expression in human adrenocortical NCI-H295R cells. / Liu, Jiangi; Li, X. D.; Vaheri, A.; Voutilainen, R.

In: Journal of Molecular Endocrinology, Vol. 33, No. 3, 12.2004, p. 651-662.

Research output: Contribution to journalArticle

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