DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress.

B. P. Chen, Y. S. Li, Y. Zhao, K. D. Chen, S. Li, J. Lao, S. Yuan, J. Y. Shyy, S. Chien

Research output: Contribution to journalArticlepeer-review

177 Scopus citations

Abstract

The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm(2). This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalPhysiological genomics
Volume7
Issue number1
DOIs
StatePublished - Oct 10 2001

ASJC Scopus subject areas

  • Physiology
  • Genetics

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