DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress.

B. P. Chen, Y. S. Li, Y. Zhao, K. D. Chen, S. Li, J. Lao, S. Yuan, J. Y. Shyy, S. Chien

Research output: Contribution to journalArticle

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Abstract

The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm(2). This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalPhysiol Genomics
Volume7
Issue number1
StatePublished - Oct 10 2001

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Microarray Analysis
Oligonucleotide Array Sequence Analysis
Endothelial Cells
Gene Expression
Genes
Transcriptome
Matrix Metalloproteinase 1
Aorta
Down-Regulation
Inflammation
Technology
Survival

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Chen, B. P., Li, Y. S., Zhao, Y., Chen, K. D., Li, S., Lao, J., ... Chien, S. (2001). DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress. Physiol Genomics, 7(1), 55-63.

DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress. / Chen, B. P.; Li, Y. S.; Zhao, Y.; Chen, K. D.; Li, S.; Lao, J.; Yuan, S.; Shyy, J. Y.; Chien, S.

In: Physiol Genomics, Vol. 7, No. 1, 10.10.2001, p. 55-63.

Research output: Contribution to journalArticle

Chen, BP, Li, YS, Zhao, Y, Chen, KD, Li, S, Lao, J, Yuan, S, Shyy, JY & Chien, S 2001, 'DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress.', Physiol Genomics, vol. 7, no. 1, pp. 55-63.
Chen, B. P. ; Li, Y. S. ; Zhao, Y. ; Chen, K. D. ; Li, S. ; Lao, J. ; Yuan, S. ; Shyy, J. Y. ; Chien, S. / DNA microarray analysis of gene expression in endothelial cells in response to 24-h shear stress. In: Physiol Genomics. 2001 ; Vol. 7, No. 1. pp. 55-63.
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