DNA polymerase β expression differences in selected human tumors and cell lines

Deepak K. Srivastava, Intisar Husain, Carlos L. Arteaga, Samuel H. Wilson

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

A long-standing question in cancer biology has been the extent to which DNA repair may be altered during the process of carcinogenesis. We have shown recently that DNA polymerase β (β-pol) provides a rate-determining function during in vitro repair of abasic sites by one of the mammalian DNA base excision repair pathways. Therefore, altered expression of β-pol during carcinogenesis could alter base excision repair and, consequently, be critical to the integrity of the mammalian genome. We examined the expression of β-pol in several cell lines and human adenocarcinomas using a quantitative immunoblotting method. In cell lines from normal breast or colon, the level of β-pol was ~ 1 ng/mg cell extract, whereas in all of the breast and colon adenocarcinoma cell lines tested, a higher level of β-pol was observed. In tissue samples, colon adenocarcinomas had a higher level of β-pol than adjacent normal mucosa. Breast adenocarcinomas exhibited a wide range of β-pol expression: one tumor had a much higher level of β-pol (286 ng/mg cell extract) than adjacent normal breast tissue, whereas another tumor had the same level of β-pol as adjacent normal tissue. Differences in β-pol expression level, from normal to elevated, were also observed with prostate adenocarcinomas. All kidney adenocarcinomas tested had a slightly lower β-pol level than adjacent normal tissue. This study reveals that the base excision repair enzyme DNA polymerase β is upregulated in some types of adenocarcinomas and cell lines, but not in others.

Original languageEnglish (US)
Pages (from-to)1049-1054
Number of pages6
JournalCarcinogenesis
Volume20
Issue number6
DOIs
StatePublished - Jan 1 1999

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DNA-Directed DNA Polymerase
Tumor Cell Line
Adenocarcinoma
DNA Repair
Breast
Cell Line
Colon
Cell Extracts
Carcinogenesis
Neoplasms
Renal Cell Carcinoma
Immunoblotting
Prostate
Mucous Membrane
Genome
DNA
Enzymes

ASJC Scopus subject areas

  • Cancer Research

Cite this

DNA polymerase β expression differences in selected human tumors and cell lines. / Srivastava, Deepak K.; Husain, Intisar; Arteaga, Carlos L.; Wilson, Samuel H.

In: Carcinogenesis, Vol. 20, No. 6, 01.01.1999, p. 1049-1054.

Research output: Contribution to journalArticle

Srivastava, Deepak K. ; Husain, Intisar ; Arteaga, Carlos L. ; Wilson, Samuel H. / DNA polymerase β expression differences in selected human tumors and cell lines. In: Carcinogenesis. 1999 ; Vol. 20, No. 6. pp. 1049-1054.
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