Abstract
The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.
Original language | English (US) |
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Pages (from-to) | 1852-1859 |
Number of pages | 8 |
Journal | ELECTROPHORESIS |
Volume | 17 |
Issue number | 12 |
DOIs | |
State | Published - 1996 |
Keywords
- Capillary electrophoresis
- Confocal fluorescence detection
- DNA sequencing
- Energy transfer primers
- Mitochondrial dna polymorphisms
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry