DNA sequencing casing a four-color confocal fluorescence capillary array scanner

I. Kheterpal, J. R. Scherer, S. M. Clark, A. Radhakrishnan, J. Ju, C. L. Ginther, G. F. Sensabaugh, R. A. Mathies

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.

Original languageEnglish (US)
Pages (from-to)1852-1859
Number of pages8
JournalElectrophoresis
Volume17
Issue number12
DOIs
StatePublished - 1996

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DNA Sequence Analysis
Color
Fluorescence
DNA
Sierra Leone
Energy Transfer
Capillary Electrophoresis
Electrophoresis
Mitochondrial DNA
Energy transfer
Lasers
Wavelength
Population

Keywords

  • Capillary electrophoresis
  • Confocal fluorescence detection
  • DNA sequencing
  • Energy transfer primers
  • Mitochondrial DNA polymorphisms

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Kheterpal, I., Scherer, J. R., Clark, S. M., Radhakrishnan, A., Ju, J., Ginther, C. L., ... Mathies, R. A. (1996). DNA sequencing casing a four-color confocal fluorescence capillary array scanner. Electrophoresis, 17(12), 1852-1859. https://doi.org/10.1002/elps.1150171209

DNA sequencing casing a four-color confocal fluorescence capillary array scanner. / Kheterpal, I.; Scherer, J. R.; Clark, S. M.; Radhakrishnan, A.; Ju, J.; Ginther, C. L.; Sensabaugh, G. F.; Mathies, R. A.

In: Electrophoresis, Vol. 17, No. 12, 1996, p. 1852-1859.

Research output: Contribution to journalArticle

Kheterpal, I, Scherer, JR, Clark, SM, Radhakrishnan, A, Ju, J, Ginther, CL, Sensabaugh, GF & Mathies, RA 1996, 'DNA sequencing casing a four-color confocal fluorescence capillary array scanner', Electrophoresis, vol. 17, no. 12, pp. 1852-1859. https://doi.org/10.1002/elps.1150171209
Kheterpal, I. ; Scherer, J. R. ; Clark, S. M. ; Radhakrishnan, A. ; Ju, J. ; Ginther, C. L. ; Sensabaugh, G. F. ; Mathies, R. A. / DNA sequencing casing a four-color confocal fluorescence capillary array scanner. In: Electrophoresis. 1996 ; Vol. 17, No. 12. pp. 1852-1859.
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