DNA sequencing using a four-color confocal fluorescence capillary array scanner

I. Kheterpal, J. R. Scherer, S. M. Clark, A. Radhakrishnan, J. Ju, C. L. Ginther, G. F. Sensabaugh, R. A. Mathies

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

The design, construction and operation of a four-color capillary array electrophoresis scanner are presented. The use of sensitive energy transfer primers facilitates four-color detection of the DNA sequencing fragments following excitation at a single laser wavelength (488 nm). This scanner collects fluorescence data from up to 25 capillaries in parallel. The resulting four-color image files are automatically reduced to four-color line plots, and a base-calling program (Sax) is used to call the sequence. The performance of this system for DNA sequencing is demonstrated by examining twelve different motifs of the hypervariable region I of human mitochondrial (mt) DNA obtained from a Sierra Leone population.

Original languageEnglish (US)
Pages (from-to)1852-1859
Number of pages8
JournalELECTROPHORESIS
Volume17
Issue number12
DOIs
StatePublished - Jan 1 1996

Keywords

  • Capillary electrophoresis
  • Confocal fluorescence detection
  • DNA sequencing
  • Energy transfer primers
  • Mitochondrial dna polymorphisms

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'DNA sequencing using a four-color confocal fluorescence capillary array scanner'. Together they form a unique fingerprint.

  • Cite this

    Kheterpal, I., Scherer, J. R., Clark, S. M., Radhakrishnan, A., Ju, J., Ginther, C. L., Sensabaugh, G. F., & Mathies, R. A. (1996). DNA sequencing using a four-color confocal fluorescence capillary array scanner. ELECTROPHORESIS, 17(12), 1852-1859. https://doi.org/10.1002/elps.1150171209