DNA sequencing using four-color capillary array electrophoresis and energy-transfer primers

Indu Kheterpal, Jingyue Ju, Arun Radhakrishnan, Gabriel S. Brandt, Charles L. Ginther, Steven M. Clark, James R. Scherer, George F. Sensabaugh, Richard A. Mathies

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Scopus citations

Abstract

A practical capillary array electrophoresis sequencer has been constructed and tested for large scale DNA sequencing. This system employs a scanning confocal optical system to detect DNA fragments electrophoresed on a capillary array. By using the recently developed energy transfer fluorescent primers, sensitive four-color detection is facilitated while exciting with just a single laser wavelength (488 nm) from an argon ion laser. Software for facile reduction of the images to four-color trace files and for automated base-calling have also been developed. This system can detect up to 25 capillaries at a time and has a raw sequencing rate of approximately 6 kilobases/hour. Applications to mitochondrial D-loop DNA sequencing are presented as a demonstration.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsGerald E. Cohn, Steven A. Soper, C.H.W. Chen
Pages204-213
Number of pages10
StatePublished - Jan 1 1996
EventUltrasensitive Biochemical Diagnostics - San Jose, CA, USA
Duration: Jan 31 1996Feb 2 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2680
ISSN (Print)0277-786X

Other

OtherUltrasensitive Biochemical Diagnostics
CitySan Jose, CA, USA
Period1/31/962/2/96

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Kheterpal, I., Ju, J., Radhakrishnan, A., Brandt, G. S., Ginther, C. L., Clark, S. M., Scherer, J. R., Sensabaugh, G. F., & Mathies, R. A. (1996). DNA sequencing using four-color capillary array electrophoresis and energy-transfer primers. In G. E. Cohn, S. A. Soper, & C. H. W. Chen (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 204-213). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2680).