Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens

Quincey Laplant, Vincent Vialou, Herbert E. Covington, Dani Dumitriu, Jian Feng, Brandon L. Warren, Ian Maze, David M. Dietz, Emily L. Watts, Sergio D. Iñiguez, Ja Wook Koo, Ezekiell Mouzon, William Renthal, Fiona Hollis, Hui Wang, Michele A. Noonan, Yanhua Ren, Amelia J. Eisch, Carlos A. Bolaños, Mohamed Kabbaj & 7 others Guanghua Xiao, Rachael L. Neve, Yasmin L. Hurd, Ronald S. Oosting, Gouping Fan, John H. Morrison, Eric J. Nestler

Research output: Contribution to journalArticle

378 Citations (Scopus)

Abstract

Despite abundant expression of DNA methyltransferases (Dnmts) in brain, the regulation and behavioral role of DNA methylation remain poorly understood. We found that Dnmt3a expression was regulated in mouse nucleus accumbens (NAc) by chronic cocaine use and chronic social defeat stress. Moreover, NAc-specific manipulations that block DNA methylation potentiated cocaine reward and exerted antidepressant-like effects, whereas NAc-specific Dnmt3a overexpression attenuated cocaine reward and was pro-depressant. On a cellular level, we found that chronic cocaine use selectively increased thin dendritic spines on NAc neurons and that DNA methylation was both necessary and sufficient to mediate these effects. These data establish the importance of Dnmt3a in the NAc in regulating cellular and behavioral plasticity to emotional stimuli.

Original languageEnglish (US)
Pages (from-to)1137-1143
Number of pages7
JournalNature Neuroscience
Volume13
Issue number9
DOIs
StatePublished - Sep 2010

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Nucleus Accumbens
Spine
Cocaine
DNA Methylation
Reward
Dendritic Spines
Methyltransferases
Antidepressive Agents
Neurons
DNA
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Laplant, Q., Vialou, V., Covington, H. E., Dumitriu, D., Feng, J., Warren, B. L., ... Nestler, E. J. (2010). Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nature Neuroscience, 13(9), 1137-1143. https://doi.org/10.1038/nn.2619

Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. / Laplant, Quincey; Vialou, Vincent; Covington, Herbert E.; Dumitriu, Dani; Feng, Jian; Warren, Brandon L.; Maze, Ian; Dietz, David M.; Watts, Emily L.; Iñiguez, Sergio D.; Koo, Ja Wook; Mouzon, Ezekiell; Renthal, William; Hollis, Fiona; Wang, Hui; Noonan, Michele A.; Ren, Yanhua; Eisch, Amelia J.; Bolaños, Carlos A.; Kabbaj, Mohamed; Xiao, Guanghua; Neve, Rachael L.; Hurd, Yasmin L.; Oosting, Ronald S.; Fan, Gouping; Morrison, John H.; Nestler, Eric J.

In: Nature Neuroscience, Vol. 13, No. 9, 09.2010, p. 1137-1143.

Research output: Contribution to journalArticle

Laplant, Q, Vialou, V, Covington, HE, Dumitriu, D, Feng, J, Warren, BL, Maze, I, Dietz, DM, Watts, EL, Iñiguez, SD, Koo, JW, Mouzon, E, Renthal, W, Hollis, F, Wang, H, Noonan, MA, Ren, Y, Eisch, AJ, Bolaños, CA, Kabbaj, M, Xiao, G, Neve, RL, Hurd, YL, Oosting, RS, Fan, G, Morrison, JH & Nestler, EJ 2010, 'Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens', Nature Neuroscience, vol. 13, no. 9, pp. 1137-1143. https://doi.org/10.1038/nn.2619
Laplant Q, Vialou V, Covington HE, Dumitriu D, Feng J, Warren BL et al. Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nature Neuroscience. 2010 Sep;13(9):1137-1143. https://doi.org/10.1038/nn.2619
Laplant, Quincey ; Vialou, Vincent ; Covington, Herbert E. ; Dumitriu, Dani ; Feng, Jian ; Warren, Brandon L. ; Maze, Ian ; Dietz, David M. ; Watts, Emily L. ; Iñiguez, Sergio D. ; Koo, Ja Wook ; Mouzon, Ezekiell ; Renthal, William ; Hollis, Fiona ; Wang, Hui ; Noonan, Michele A. ; Ren, Yanhua ; Eisch, Amelia J. ; Bolaños, Carlos A. ; Kabbaj, Mohamed ; Xiao, Guanghua ; Neve, Rachael L. ; Hurd, Yasmin L. ; Oosting, Ronald S. ; Fan, Gouping ; Morrison, John H. ; Nestler, Eric J. / Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. In: Nature Neuroscience. 2010 ; Vol. 13, No. 9. pp. 1137-1143.
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