Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice

Haruhiko Koseki, Hidefumi Asano, Tsuneyoshi Inaba, Nobumoto Miyashita, Kazuo Moriwaki, Kirsten Fischer Lindahl, Yoko Mizutani, Kenji Imai, Masaru Taniguchi

Research output: Contribution to journalArticle

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Abstract

A distinctive variable region 14-positive (V14+) α chain (Vα14+) of the T-cell antigen receptor is predominantly expressed in multiple mouse subspecies. The Vα14 family has two members, Vα4.1 and Vα14.2, which differ by only three amino acids at positions 50-52. Based on the EcoRI restriction fragment length polymorphism of the gene encoding Vα14, mice can be divided into three groups: type I with an 11.2-kilobase (kb) fragment, type II with a 2.0-kb fragment, and type III with the 2.0-kb and 11.2-kb fragments. Usage of Vα14-Jα281, where Jα281 is an α-chain joining segment, with a one-base N region dominates at the level of 0.02-1.5% of α chains in all laboratory strains, Mus musculus castaneus, and Mus musculus domesticus but not in Mus musculus molossinus, Mus musculus musculus, and Mus spicilegus samples. The preferential Vα14-Jα281 expression seems to be due to positive selection because the V-J junctional region is always glycine, despite the ability of the Vα14 gene to associate with Ja other than Jα281. As Vα14-Jα281 expression is independent of known major histocompatibility complex antigens, including H-2, TLA, Qa, and HMT, the selecting ligand must be a monomorphic molecule of the mouse, expressed in a subspecies-specific manner. Additional observations, such as the expression of homogeneous Vα14-Jα281 in athymic mice, suggest that the positive selection of Vα14+ T cells occurs extrathymically.

Original languageEnglish (US)
Pages (from-to)7518-7522
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number17
StatePublished - 1991

Fingerprint

T-Cell Antigen Receptor
Histocompatibility Antigens
Major Histocompatibility Complex
Nude Mice
Restriction Fragment Length Polymorphisms
Glycine
Genes
Ligands
T-Lymphocytes
Amino Acids

Keywords

  • Extrathymic-positive selection
  • Homogeneous variable-joining region junction
  • Variable region 14 genes
  • Wild mice

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Koseki, H., Asano, H., Inaba, T., Miyashita, N., Moriwaki, K., Lindahl, K. F., ... Taniguchi, M. (1991). Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice. Proceedings of the National Academy of Sciences of the United States of America, 88(17), 7518-7522.

Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice. / Koseki, Haruhiko; Asano, Hidefumi; Inaba, Tsuneyoshi; Miyashita, Nobumoto; Moriwaki, Kazuo; Lindahl, Kirsten Fischer; Mizutani, Yoko; Imai, Kenji; Taniguchi, Masaru.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 17, 1991, p. 7518-7522.

Research output: Contribution to journalArticle

Koseki, H, Asano, H, Inaba, T, Miyashita, N, Moriwaki, K, Lindahl, KF, Mizutani, Y, Imai, K & Taniguchi, M 1991, 'Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 17, pp. 7518-7522.
Koseki, Haruhiko ; Asano, Hidefumi ; Inaba, Tsuneyoshi ; Miyashita, Nobumoto ; Moriwaki, Kazuo ; Lindahl, Kirsten Fischer ; Mizutani, Yoko ; Imai, Kenji ; Taniguchi, Masaru. / Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 17. pp. 7518-7522.
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