Donor age negatively affects the immunoregulatory properties of both adipose and bone marrow derived mesenchymal stem cells

Lehao W. Wu, Yen Ling Wang, Joani M. Christensen, Saami Khalifian, Stefan Schneeberger, Giorgio Raimondi, Damon S. Cooney, W. P.Andrew Lee, Gerald Brandacher

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Purpose: Age negatively impacts the biologic features of mesenchymal stem cells (MSCs), including decreased expansion kinetics and differentiation potential. Clinically, donor-age may be within a wide spectrum; therefore, investigation of the role of donor's age on immunoregulatory potential is of critical importance to translate stem cell therapies from bench to bedside. Methods: Adipose and bone marrow derived MSCs (ASCs and BMSCs) were isolated in parallel from Lewis and Brown Norway rats of young (less than 4-week old) and senior groups (older than 15-month). The presentation of cells and time required for growth to 90% confluence was recorded. FACS sorting based on the expression of CD90 and CD29 double positive and CD45 CD11 double negative quantified the proportions of MSCs. After expansion, ASCs and BMSCs from different age groups were co-cultured in mixed lymphocyte reaction (MLR; Lewis vs. Brown Norway) assays. The suppression of CD3+CD4+ and CD3+CD8+ T cell populations by different sources of MSCs were compared. Results: The kinetics of cell growth was slower in old animals (17.3±2days) compared with young animals (8.8±3days), and cell morphology was irregular and enlarged in the senior groups. The yield of MSCs by FACS sorting was significantly higher in young groups compared to senior groups (p<0.02). With regard to immunoregulatory potential, senior ASCs failed to induce any CD3+CD4+ T cell suppression (p>0.05). In addition, young BMSCs-induced suppression was more prominent than seniors (p<0.05). Conclusions: Donor age should be taken into consideration when using recipient MSC of either bone marrow or adipose origin in clinical applications.

Original languageEnglish (US)
Pages (from-to)122-127
Number of pages6
JournalTransplant Immunology
Volume30
Issue number4
DOIs
StatePublished - May 2014
Externally publishedYes

Keywords

  • Adipose tissue
  • Age
  • Bone marrow
  • Immunoregulation
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Transplantation

Fingerprint

Dive into the research topics of 'Donor age negatively affects the immunoregulatory properties of both adipose and bone marrow derived mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this