Double isotype production by a neoplastic B cell line. II. Allelically excluded production of μ and γ1 heavy chains without C(H) gene rearrangement

Y. W. Chen, C. Word, V. Dev, J. W. Uhr, E. S. Vitetta, P. W. Tucker

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Abstract

In our accompanying paper, we described a switch variant (BCL1.2.58) that expresses membrane and secreted forms of IgM and IgG1. Both IgM and IgG1 share the same idiotype and use the same VDJ rearrangement. Here, a detailed Southern blot analysis of the entire constant region of the Ig heavy chain (Ig C(H)) locus of parental (BCL1.B1) and variants (BCL1.B2) DNA showed no detectable rearrangement. Similar analysis of the J(H)-Cμ region led to the conclusion that two heavy chain alleles present in the IgM/IgG1-producing variants carried the same VDJ rearrangement but differed in their 3' flanking regions. One chromosome 12 did not carry any Ig C(H) genes, whereas, the other chromosome 12 carried one copy of C(H) genes. In BCL1.B1, however, each of the chromosome 12 alleles carried a full copy of C(H) genes. Karyotypic analysis confirmed the presence of two translocated t(12;16) chromosomes in both BCL1.2.58 and BCL1.B1 cells, with a break 5' to the V(H) locus at the distal region (12F2) of chromosome 12, and at the proximal region below the centromere (16B3) of chromosome 16. We conclude that double production of IgM and IgG1 in BCL1.B2 is accomplished by transcription of the corresponding C(H) genes in germline configuration using a single VDJ on the same chromosome 12.

Original languageEnglish (US)
Pages (from-to)562-579
Number of pages18
JournalJournal of Experimental Medicine
Volume164
Issue number2
StatePublished - 1986

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Chromosomes, Human, Pair 12
Gene Rearrangement
B-Lymphocytes
Cell Line
Immunoglobulin M
Immunoglobulin G
Chromosomes, Human, Pair 16
Immunoglobulin Heavy Chains
Genes
Alleles
3' Flanking Region
Centromere
Southern Blotting
Membranes
DNA

ASJC Scopus subject areas

  • Immunology

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Double isotype production by a neoplastic B cell line. II. Allelically excluded production of μ and γ1 heavy chains without C(H) gene rearrangement. / Chen, Y. W.; Word, C.; Dev, V.; Uhr, J. W.; Vitetta, E. S.; Tucker, P. W.

In: Journal of Experimental Medicine, Vol. 164, No. 2, 1986, p. 562-579.

Research output: Contribution to journalArticle

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AU - Uhr, J. W.

AU - Vitetta, E. S.

AU - Tucker, P. W.

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N2 - In our accompanying paper, we described a switch variant (BCL1.2.58) that expresses membrane and secreted forms of IgM and IgG1. Both IgM and IgG1 share the same idiotype and use the same VDJ rearrangement. Here, a detailed Southern blot analysis of the entire constant region of the Ig heavy chain (Ig C(H)) locus of parental (BCL1.B1) and variants (BCL1.B2) DNA showed no detectable rearrangement. Similar analysis of the J(H)-Cμ region led to the conclusion that two heavy chain alleles present in the IgM/IgG1-producing variants carried the same VDJ rearrangement but differed in their 3' flanking regions. One chromosome 12 did not carry any Ig C(H) genes, whereas, the other chromosome 12 carried one copy of C(H) genes. In BCL1.B1, however, each of the chromosome 12 alleles carried a full copy of C(H) genes. Karyotypic analysis confirmed the presence of two translocated t(12;16) chromosomes in both BCL1.2.58 and BCL1.B1 cells, with a break 5' to the V(H) locus at the distal region (12F2) of chromosome 12, and at the proximal region below the centromere (16B3) of chromosome 16. We conclude that double production of IgM and IgG1 in BCL1.B2 is accomplished by transcription of the corresponding C(H) genes in germline configuration using a single VDJ on the same chromosome 12.

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