Double-stranded DNA bacteriophage prohead protease is homologous to herpesvirus protease

Hua Cheng, Nan Shen, Jimin Pei, Nick V. Grishin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Double-stranded DNA bacteriophages and herpesviruses assemble their heads in a similar fashion; a preformed precursor called a prohead or procapsid undergoes a conformational transition to give rise to a mature head or capsid. A virus-encoded prohead or procapsid protease is often required in this maturation process. Through computational analysis, we infer homology between bacteriophage prohead proteases (MEROPS families U9 and U35) and herpesvirus protease (MEROPS family S21), and unify them into a procapsid protease superfamily. We also extend this superfamily to include an uncharacterized cluster of orthologs (COG3566) and many other phage or bacteria-encoded hypothetical proteins. On the basis of this homology and the herpesvirus protease structure and catalytic mechanism, we predict that bacteriophage prohead proteases adopt the herpesvirus protease fold and exploit a conserved Ser and His residue pair in catalysis. Our study provides further support for the proposed evolutionary link between dsDNA bacteriophages and herpesviruses.

Original languageEnglish (US)
Pages (from-to)2260-2269
Number of pages10
JournalProtein Science
Volume13
Issue number8
DOIs
StatePublished - Aug 2004

Fingerprint

Bacteriophages
Herpesviridae
Peptide Hydrolases
DNA
Head
Capsid
Catalysis
Viruses
Catalyst supports
Bacteria

Keywords

  • dsDNA bacteriophage
  • Evolution
  • Gene organization
  • Homology detection
  • MEROPS
  • Prohead protease
  • Structure prediction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Double-stranded DNA bacteriophage prohead protease is homologous to herpesvirus protease. / Cheng, Hua; Shen, Nan; Pei, Jimin; Grishin, Nick V.

In: Protein Science, Vol. 13, No. 8, 08.2004, p. 2260-2269.

Research output: Contribution to journalArticle

Cheng, Hua ; Shen, Nan ; Pei, Jimin ; Grishin, Nick V. / Double-stranded DNA bacteriophage prohead protease is homologous to herpesvirus protease. In: Protein Science. 2004 ; Vol. 13, No. 8. pp. 2260-2269.
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