double-time is a novel Drosophila clock gene that regulates period protein accumulation

Jeffrey L. Price, Justin Blau, Adrian Rothenfluh, Marla Abodeely, Brian Kloss, Michael W. Young

Research output: Contribution to journalArticle

597 Citations (Scopus)

Abstract

We have isolated three alleles of a novel Drosophila clock gene, double- time (dbt). Short- (dbt(S)) and longperiod (dbt(L)) mutants alter both behavioral rhythmicity and molecular oscillations from previously identified clock genes, period and timeless. A third allele, dbt(P), causes pupal lethality and eliminates circadian cycling of per and tim gene products in larvae. In dbt(P) mutants, PER proteins constitutively accumulate, remain hypophosphorylated, and no longer depend on TIM proteins for their accumulation. We propose that the normal function of DOUBLETIME protein is to reduce the stability and thus the level of accumulation of monomeric PER proteins. This would promote a delay between per/tim transcription and PER/TIM complex function, which is essential for molecular rhythmicity.

Original languageEnglish (US)
Pages (from-to)83-95
Number of pages13
JournalCell
Volume94
Issue number1
DOIs
StatePublished - Jul 10 1998

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Period Circadian Proteins
Drosophila
Clocks
Genes
Periodicity
Proteins
Alleles
Transcription
Mutant Proteins
Larva

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

double-time is a novel Drosophila clock gene that regulates period protein accumulation. / Price, Jeffrey L.; Blau, Justin; Rothenfluh, Adrian; Abodeely, Marla; Kloss, Brian; Young, Michael W.

In: Cell, Vol. 94, No. 1, 10.07.1998, p. 83-95.

Research output: Contribution to journalArticle

Price, Jeffrey L. ; Blau, Justin ; Rothenfluh, Adrian ; Abodeely, Marla ; Kloss, Brian ; Young, Michael W. / double-time is a novel Drosophila clock gene that regulates period protein accumulation. In: Cell. 1998 ; Vol. 94, No. 1. pp. 83-95.
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